Nesprin 1 (SYNE1) Human shRNA Plasmid Kit (Locus ID 23345)

CAT#: TL301288

SYNE1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL301288A virus into HEK293 cells. TL301288A virus was prepared using lenti-shRNA TL301288A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL301288B virus into HEK293 cells. TL301288B virus was prepared using lenti-shRNA TL301288B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL301288C virus into HEK293 cells. TL301288C virus was prepared using lenti-shRNA TL301288C and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL301288D virus into HEK293 cells. TL301288D virus was prepared using lenti-shRNA TL301288D and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 23345
• Synonyms: 8B; ARCA1; C6orf98; CPG2; dJ45H2.2; DKFZp781J13156; EDMD4; FLJ30878; FLJ41140; KIAA0796
• Vector: pGFP-C-shLenti
• E. coli Selection: Chloramphenicol
• Mammalian Cell Selection: Puromycin
• Format: Lentiviral plasmids
• Kit Components: SYNE1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 23345). 5µg purified plasmid DNA per construct
  Non-effective 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
• RefSeq: NM_015293, NM_033071, NM_133650, NM_182961, NM_001347701, NM_001347702, BC028616, BC039121, BC090927, BC150289
• Summary: This gene encodes a spectrin repeat containing protein expressed in skeletal and smooth muscle, and peripheral blood lymphocytes, that localizes to the nuclear membrane. Mutations in this gene have been associated with autosomal recessive spinocerebellar ataxia 8, also referred to as autosomal recessive cerebellar ataxia type 1 or recessive ataxia of Beauce. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).