RNF20 Human shRNA Plasmid Kit (Locus ID 56254)

CAT#: TL301942

RNF20 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector


USD 815.00

In Stock*

Size
    • 5 ug/vial

Product Images

Frequently bought together (1)
Lateral flow testing sticks used for the semi-quantitative detection of the lentiviral p24 protein, 20 reactions
    • 1 kit

USD 428.00

Other products for "RNF20"

Specifications

Product Data
Locus ID 56254
Synonyms BRE1; BRE1A; hBRE1
Vector pGFP-C-shLenti
Format Lentiviral plasmids
Kit Components RNF20 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 56254). 5µg purified plasmid DNA per construct
Non-effective 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq NM_019592, BC110584, BC016691, BC032618, BC063115, BC110585, BC152309
Summary The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. The protein encoded by this human gene is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. This protein acts as a putative tumor suppressor and positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. In contrast, this protein also suppresses the expression of several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor. This gene selectively suppresses the expression of some genes by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS). [provided by RefSeq, Feb 2012]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.