GDNF Receptor alpha 1 (GFRA1) Human shRNA Plasmid Kit (Locus ID 2674)

CAT#: TL304358

GFRA1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL304358A virus into HEK293 cells. TL304358A virus was prepared using lenti-shRNA TL304358A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL304358B virus into HEK293 cells. TL304358B virus was prepared using lenti-shRNA TL304358B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL304358C virus into HEK293 cells. TL304358C virus was prepared using lenti-shRNA TL304358C and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL304358D virus into HEK293 cells. TL304358D virus was prepared using lenti-shRNA TL304358D and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 2674
• Synonyms: GDNFR; GDNFRA; GFR-ALPHA-1; RET1L; RETL1; TRNR1
• Vector: pGFP-C-shLenti
• E. coli Selection: Chloramphenicol
• Mammalian Cell Selection: Puromycin
• Format: Lentiviral plasmids
• Kit Components: GFRA1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 2674). 5µg purified plasmid DNA per construct
  Non-effective 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
• RefSeq: NM_001145453, NM_005264, NM_145793, NM_001348096, NM_001348097, NM_001348098, NM_001348099, BC014962, BM675677
• Summary: 'This gene encodes a member of the glial cell line-derived neurotrophic factor receptor (GDNFR) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature receptor. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. This receptor is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]'
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).