CTIP2 (BCL11B) Human shRNA Plasmid Kit (Locus ID 64919)

CAT#: TL306424

BCL11B - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL306424A virus into HEK293 cells. TL306424A virus was prepared using lenti-shRNA TL306424A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL306424B virus into HEK293 cells. TL306424B virus was prepared using lenti-shRNA TL306424B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL306424C virus into HEK293 cells. TL306424C virus was prepared using lenti-shRNA TL306424C and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL306424D virus into HEK293 cells. TL306424D virus was prepared using lenti-shRNA TL306424D and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 64919
• Synonyms: ATL1; ATL1-alpha; ATL1-beta; ATL1-delta; ATL1-gamma; CTIP-2; CTIP2; hRIT1-alpha; IDDFSTA; IMD49; RIT1; ZNF856B
• Vector: pGFP-C-shLenti
• E. coli Selection: Chloramphenicol
• Mammalian Cell Selection: Puromycin
• Format: Lentiviral plasmids
• Kit Components: BCL11B - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 64919). 5µg purified plasmid DNA per construct
  Non-effective 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
• RefSeq: NM_001282237, NM_001282238, NM_022898, NM_138576, BC156139, NM_001282237.2
• Summary: This gene encodes a C2H2-type zinc finger protein and is closely related to BCL11A, a gene whose translocation may be associated with B-cell malignancies. Although the specific function of this gene has not been determined, the encoded protein is known to be a transcriptional repressor, and is regulated by the NURD nucleosome remodeling and histone deacetylase complex. Four alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Aug 2013]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).