SOX2 Human shRNA Plasmid Kit (Locus ID 6657)

CAT#: TL309173

SOX2 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL309173A virus into HEK293 cells. TL309173A virus was prepared using lenti-shRNA TL309173A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL309173B virus into HEK293 cells. TL309173B virus was prepared using lenti-shRNA TL309173B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL309173C virus into HEK293 cells. TL309173C virus was prepared using lenti-shRNA TL309173C and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL309173D virus into HEK293 cells. TL309173D virus was prepared using lenti-shRNA TL309173D and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 6657
• Synonyms: ANOP3; MCOPS3
• Vector: pGFP-C-shLenti
• E. coli Selection: Chloramphenicol
• Mammalian Cell Selection: Puromycin
• Format: Lentiviral plasmids
• Kit Components: SOX2 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 6657). 5µg purified plasmid DNA per construct
  Non-effective 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
• RefSeq: BC013923, NM_003106, BM668019, BM722297
• Summary: 'This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]'
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).