Syndecan 1 (SDC1) Human shRNA Plasmid Kit (Locus ID 6382)

CAT#: TL309598

SDC1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL309598A virus into HEK293 cells. TL309598A virus was prepared using lenti-shRNA TL309598A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL309598B virus into HEK293 cells. TL309598B virus was prepared using lenti-shRNA TL309598B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL309598C virus into HEK293 cells. TL309598C virus was prepared using lenti-shRNA TL309598C and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL309598D virus into HEK293 cells. TL309598D virus was prepared using lenti-shRNA TL309598D and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 6382
• Synonyms: CD138; SDC; SYND1; syndecan
• Vector: pGFP-C-shLenti
• E. coli Selection: Chloramphenicol
• Mammalian Cell Selection: Puromycin
• Format: Lentiviral plasmids
• Kit Components: SDC1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 6382). 5µg purified plasmid DNA per construct
  Non-effective 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
• RefSeq: NM_001006946, NM_002997, BC008765, BM785035, BM910157
• Summary: The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).