MIA2 Human siRNA Oligo Duplex (Locus ID 4253)

CAT#: SR320967

MIA2 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

Specifications

Product Data

• Purity: HPLC purified
• Quality Control: Tested by ESI-MS
• Sequences: Available with shipment
• Components: MIA2 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 4253)
  Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol
  Included - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml
• Stability: One year from date of shipment when stored at -20°C.
• Number of Transfections: Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).
• Note: Single siRNA duplex (10nmol) can be ordered.

Reference Data

• RefSeq: NM_001247988, NM_001247989, NM_001247990, NM_005930, NM_054024, NM_203354, NM_203355, NM_203356, NM_203357, NM_001329214, NM_001354137, NM_001354138, NM_001354139, NM_001354140, NM_001354141, NM_001354142, NM_001354143, NM_001354144, NM_001354145, NM_001354146, NM_001354147, NM_001354148, NM_001354149, NM_001354150, NM_001354151, NM_001354152, NM_001354153, NM_001354154, NM_001354155, NM_001354156, NM_001354157, NR_148721, NR_148722, NR_148723
• Synonyms: CTAGE5; MEA6; MGEA; MGEA6; MGEA11; TALI
• Summary: 'This gene encodes s receptor in the endoplasmic reticulum, which plays a role in the export of large pre-chylomicrons and pre-very low density lipoproteins (pre-VLDLs). Three major classes of transcripts are generated from this gene- melanoma inhibitory activity 2-specific transcripts, cTAGE family member 5-specific transcripts and transcripts that include exons from both these transcript species (TANGO1-like or TALI). Additionally, alternative splicing in these transcripts results in multiple transcript variants encoding multiple isoforms. [provided by RefSeq, Sep 2016]'
• Performance Guaranteed: OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency.
  
  For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).