pT7-Guide-IVT

CAT#: GE100025

pT7-Guide-IVT vector for target sequence cloning; IVT to produce gRNA, (10ug)

Product Images

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Specifications

Product Data

• Function: CRISPR/Cas9 T7 Vectors
• Features:

  T7 driven gRNA cloning vector

  • gRNA can be produced via in vitro transcription after the target sequence being cloned
  • BsmB I sites are used for target sequence cloning
  • For mRNA mediated genome editing via microinjection or mRNA transfection, both Cas9 mRNA and gRNA transcript are needed. T7 driven Cas9 mRNA can be produced from a separate vector, SKU GE100014
  • M13 forward sequencing primer can be used to sequence the target sequence after cloning.

  Download vector sequence