Secondary Antibodies

View as table Download

Rat IgG (H+L chain, adsorbed) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/1,500.
Conjugation HRP
Immunogen Rat IgG whole molecules.

Hamster IgG (adsorbed) goat polyclonal antibody, Biotin

Applications ELISA: 1/1000-1/5000.
Flow Cytometry: Use 50 µl of 1/25-1/50 diluted antibody to label 106 cells in 100 µl.
Reactivities Hamster
Conjugation Biotin
Immunogen Hamster IgG

Porcine IgA goat polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000. 
Western Blot: 1/1000-1/10000. 
Immunohistochemistry on Frozen Sections: 1/100-1/1000.
Reactivities Porcine
Conjugation AP
Immunogen Purified Porcine IgA.

Porcine IgA goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on Frozen Sections: 1/200-1/2000.
Reactivities Porcine
Conjugation FITC
Immunogen Purified Porcine IgA

Rat IgA goat polyclonal antibody, HRP

Applications ELISA: 1:10,000.
Western Blot: 1:1,000 - 10,000.
Reactivities Rat
Conjugation HRP

Rat IgG1 goat polyclonal antibody, HRP

Applications ELISA: 1/10,000.
Western Blot: 1/1,000-1/10,000.
Reactivities Rat
Conjugation HRP

Rat IgG2c goat polyclonal antibody, HRP

Applications ELISA: 1:10000.
Western Blot: 1:1000 - 1:10000.
Reactivities Rat
Conjugation HRP

Rat IgM goat polyclonal antibody, HRP

Applications ELISA: 1:10,000 - 1:100,000.
Western Blot: 1:1000 - 1:10,000.
Reactivities Rat
Conjugation HRP

Mouse IgG (adsorbed) goat polyclonal antibody, AP

Applications ELISA: 1/200-1/2500.
Western Blot: 1/100-1/500.
Reactivities Mouse
Conjugation AP
Immunogen Whole Mouse IgG

Mouse IgG (adsorbed) goat polyclonal antibody, HRP

Applications ELISA: 1/10000-1/100000.
Western Blot: 1/1000-1/10000.
Reactivities Mouse
Conjugation HRP
Immunogen Antisera to Mouse IgG were raised by repeated immunisations of Goats with highly purified antigen.

Mouse IgG2c goat polyclonal antibody, FITC

Applications Flow Cytometry.
Reactivities Mouse
Conjugation FITC

Human IgE goat polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Western Blot: 1/1000-1/5000.
Reactivities Human, Monkey
Conjugation AP

Human IgE goat polyclonal antibody, Biotin

Applications ELISA: 1/10,000-1/100,000. 
Western Blot: 1/10,000-1/100,000.
Reactivities Human, Monkey
Conjugation Biotin
Immunogen Native human IgE

Rabbit IgA goat polyclonal antibody, Purified

Applications ELISA: 1/100 - 1/1000.
Immunodiffusion.
Reactivities Rabbit
Immunogen Purified rabbit IgA

Bovine IgG (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgG in bovine serum or other body fluids or to determine its concentration.
To prepare an Immunoadsorbent for the purification of Bovine IgG from serum or plasma.
Recommended Dilutions:
Immunoelectrophoresis: Use 2 µl serum or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
Precipitin titre: 1/64 when tested against pooled normal bovine serum in agar-block Immunodiffusion titration.
Reactivities Bovine
Immunogen Highly purified normal IgG isolated from pooled Bovine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Chicken IgM (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Recommended Working Dilutions:
Histochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
Antibody titre: Precipitin titre 1/32 when tested against pooled normal chicken serum in agar-block immunodiffusion titration.
Reactivities Chicken
Immunogen Purified IgM isolated from Chicken serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgD (Fc specific) goat polyclonal antibody, Azide Free

Applications This antiserum is intended to detect and identify IgD in serum or other body fluids using the Immunofixation technique. Like Immunoelectrophoresis Immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization.
Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins.
Antibody titre: Precipitin titre not less than 1:16 when tested against an appropriate reference in agar immunodiffusion block titration.
Reactivities Human
Immunogen Highly purified monoclonal IgD isolated from Human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgE (Fc specific) goat polyclonal antibody, Azide Free

Applications This antiserum is intended to detect and identify IgE in serum or other body fluids using the immunofixation technique. Like immunoelectrophoresis immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization.
Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.
Antibody titre: Precipitin titre not less than 1/16 when tested against an appropriate reference in agar immunodiffusion block titration.
Reactivities Human
Immunogen Highly purified monoclonal IgE isolated from human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Canine IgA (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in dog serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/5000.
Reactivities Canine
Conjugation Biotin
Immunogen Purified normal IgA isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Canine IgG (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in immunocytochemical and immunohistochemical use for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in dog serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/5000.
Reactivities Canine
Conjugation Biotin
Immunogen Purified normal IgG isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Canine IgM (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in immunocytochemical and immunohistochemical use for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in dog serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/4000.
Reactivities Canine
Conjugation Biotin
Immunogen Purified normal IgM isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, BGAL

Applications ELISA: 1/500.
Immunohistochemistry.
Reactivities Mouse
Conjugation BGAL
Immunogen Pooled antisera from Goats hyperimmunized with Mouse IgM, IgG and IgA paraproteins.

Porcine IgA goat polyclonal antibody, Biotin

Applications ELISA: 1/10000-1/100000. 
Western Blot: 1/10000-1/100000
Reactivities Porcine
Conjugation Biotin
Immunogen Purified Porcine IgA

Porcine IgA goat polyclonal antibody, Aff - Purified

Applications ELISA: 1/100-1/1000.
Immunodiffusion.
Reactivities Porcine
Immunogen Purified Porcine IgA.

Guinea pig IgG (H+L chain) goat polyclonal antibody, HRP, Purified

Applications ELISA (1/1000-1/10,000).
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.
Reactivities Guinea Pig
Conjugation HRP
Immunogen Highly purified Guinea pig IgG (H&L)

Goat Monoclonal Anti-Rabbit IgG Fab antibody, clone RMG01

Applications ELISA: 0.01ug/mL – 0.5ug/mL; ICC/IHC: 0.5ug/mL-2ug/mL; WB (non-reduced): 0.1ug/mL-0.5ug/mL
Reactivities Rabbit IgG Fab
Conjugation Unconjugated

Goat Monoclonal Anti-Rabbit IgG Fc antibody, clone RMG02

Applications ELISA: 0.01ug/mL – 0.5ug/mL, ICC/IHC: 0.5ug/mL-2ug/mL, WB (non-reduced): 0.1ug/mL-0.5ug/mL
Reactivities Rabbit IgG Fc
Conjugation Unconjugated

Mouse IgG2b goat polyclonal antibody

Applications ELISA.
Reactivities Mouse
Immunogen Mouse IgG2b paraproteins

Hamster IgG (F(ab)2 specific) goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/100 - 1/200.
Reactivities Hamster
Conjugation FITC
Immunogen Hamster IgG

Hamster IgG (F(ab)2 specific) goat polyclonal antibody, Purified

Applications ELISA: 1/1000-1/2000.
Reactivities Hamster, Reptiles
Immunogen Hamster IgG

Mouse IgM goat polyclonal antibody, APC

Applications Immunofluorescence.
Reactivities Mouse
Conjugation APC
Immunogen Mouse IgM paraproteins

Mouse IgE goat polyclonal antibody, AP

Applications ELISA: 1:100 - 1:1000.
Reactivities Mouse
Conjugation AP

Mouse IgE goat polyclonal antibody, HRP

Applications ELISA: 1:100 - 1:1000.
Reactivities Mouse
Conjugation HRP

Mouse IgE goat polyclonal antibody, Purified

Applications ELISA: 1:100 - 1:1000.
Reactivities Mouse

Rat IgG1 goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 50 µl of 1/400 diluted antibody to label 1 x 106 cells in 100 µl.
Reactivities Rat
Conjugation FITC

Rat IgG2a goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/500.
Reactivities Rat
Conjugation FITC

Mouse IgG (adsorbed) goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 50 µl of 1/50-1/200 diluted antibody to label 1x106 cells in 100 µl.
Reactivities Mouse
Conjugation FITC

Mouse IgG1 goat polyclonal antibody, PE

Applications Flow Cytometry: 1/20 - 1/30.
Reactivities Mouse
Conjugation PE
Immunogen Mouse IgG1 paraproteins

Mouse IgG2a goat polyclonal antibody, PE

Applications Flow Cytometry: 1/20 - 1/30.
Reactivities Mouse
Conjugation PE
Immunogen Mouse IgG2a paraproteins

Mouse IgG2b goat polyclonal antibody, PE

Applications Flow Cytometry: Use 10 µl of 1/20-1/30 diluted antibody to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation PE
Immunogen Mouse IgG2b paraproteins.

Mouse IgG2c goat polyclonal antibody

Applications ELISA: 1/1000 - 1/10000.
Reactivities Mouse

Mouse IgG2c goat polyclonal antibody, HRP

Applications ELISA: 1/10000-1/100000.
Reactivities Mouse
Conjugation HRP

Human IgD (heavy chain) goat polyclonal antibody, Purified

Applications ELISA.
Reactivities Human
Immunogen Human IgD paraproteins.

Rabbit IgA goat polyclonal antibody, AP

Applications ELISA: 1/100-1/1000.
Reactivities Rabbit
Conjugation AP
Immunogen Purified Rabbit IgA

Rabbit IgA goat polyclonal antibody, HRP

Applications ELISA: 1/10000-1/100000.
Reactivities Rabbit
Conjugation HRP
Immunogen Purified rabbit IgA

Turkey IgG (H+L chain) goat polyclonal antibody, AP

Applications ELISA: 1/2,000-1/4,000.
Conjugation AP

Turkey IgG (H+L chain) goat polyclonal antibody, HRP

Applications ELISA: 1/4,000-1/8,000.
Conjugation HRP

Mouse IgG3 goat polyclonal antibody, Purified

Applications ELISA.
Reactivities Mouse
Immunogen Mouse IgG3 paraproteins.

Mouse IgG goat polyclonal antibody, FITC, Aff - Purified

Applications Immunofluorescence: 1/500-1/1000. 
Immunohistochemistry.
Reactivities Mouse
Conjugation FITC
Immunogen Purified Mouse IgG fraction

Canine IgM goat polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Reactivities Canine
Conjugation AP
Immunogen Purified Canine IgM.