Secondary Antibodies

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Lambda light chain, mouse anti human, clone λ-1 Biotin

Applications Tested for immunohistochemistry (IHC) Approximate working dilution for IHC: Frozen sections: 0.125µg/ml (1:1600) Paraffin sections: Has been described to work with proteinase K pretreatment. Optimal dilutions should be determined by the end user. Suggested positive control: Human tonsil.
Reactivities Human
Conjugation Biotin

Kappa light chain, mouse anti human, clone κ -117 Biotin

Applications Tested for immunohistochemistry (IHC) Approximate working dilution for IHC: Frozen sections: 0.25µg/ml (1:800) Paraffin sections: Has been described to work with proteinase K pretreatment. Optimal dilutions should be determined by the end user. Suggested positive control: Human tonsil.
Reactivities Human
Conjugation Biotin

Human IgG4 (Fc specific) mouse monoclonal antibody, clone BDI541, Purified

Applications ELISA.
Western Blot.
Immunohistochemistry on frozen sections.
Reactivities Human

Human IgA1 (alpha chain) mouse monoclonal antibody, clone B3506B4, AP

Applications Immunohistochemistry on frozen sections.
ELISA.
Western Blot.
Immunoblotting.
Reactivities Human
Conjugation AP

Human IgA1 (alpha chain) mouse monoclonal antibody, clone B3506B4, Purified

Applications ELISA.
Western Blot.
Immunoblotting. 
Immunohistochemistry on Frozen Sections.
Reactivities Human

Human IgG4 (pFC) mouse monoclonal antibody, clone HP6023 (also known as HO6023), Purified

Applications ELISA.
Western Blot.
Immunoblotting. 
Immunohistochemistry on Frozen Sections.
Reactivities Human

Porcine IgM mouse monoclonal antibody, clone 206-2, Aff - Purified

Applications ELISA: 0.1 µg/ml (1/4000).
Immunoprecipitation.
Immunohistochemistry on Frozen Sections: 0.2 µg/ml (1/2000).
Immunohistochemistry on Paraffin Sections (Does not react on routinely processed Paraffin Section).
Does not react in Western Blot with reduced and denatured samples.
Reactivities Porcine

Human IgG (heavy chain) mouse monoclonal antibody, clone BDI412, Azide Free

Applications ELISA.
Western Blot.
Immunohistochemistry on frozen sections.
Reactivities Human

Human IgM mouse monoclonal antibody, clone ICL-931, Purified

Applications Dot Blot.
ELISA.
Radioimmunoassay.
Immunohistochemistry on frozen sections: 1/40.
Reactivities Human

Human IgG mouse monoclonal antibody, clone EFE-565, HRP, Purified

Applications Dot Blot.
ELISA.
Radioimmunoassay.
Immunohistochemistry on Frozen Sections: 1/40.
Reactivities Human
Conjugation HRP

Human IgG mouse monoclonal antibody, clone EFE-565, Purified

Applications ELISA.
Dot Blot.
Radioimmunoassay.
Immunohistochemistry on frozen sections: 1/40.
Reactivities Human

Human IgA mouse monoclonal antibody, clone BFG-267, HRP, Purified

Applications ELISA: 1/1000.
Radioimmunoassay.
Dot Blot: 1/100.
Immunohistochemistry on frozen sections: 1/40.
Reactivities Human
Conjugation HRP

Human IgA mouse monoclonal antibody, clone BFG-267, Purified

Applications ELISA.
Radioimmunoassay.
Dot Blot.
Immunohistochemistry on frozen sections: 1/40.
Reactivities Human

IGHG1 (heavy chain) mouse monoclonal antibody, clone ICO-97, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT.
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human

IGHG1 (heavy chain) mouse monoclonal antibody, clone ICO-97, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT.
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human

Goat IgG (H+L chain) mouse polyclonal antibody, Serum

Applications ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunochemistry: 1/1,000-1/5,000.
Reactivities Goat
Immunogen Goat IgG whole molecule.

Human IgG1 (Fc specific) mouse monoclonal antibody, clone 8c/6-39 (HP6091), Purified

Applications ELISA.
Immunodiffusion.
Immunohistochemistry on Frozen Sections.
Not suitable for Paraffin Sections.
Reactivities Human

Human IgG2 (F(ab)2 specific) mouse monoclonal antibody, clone HP6014, Purified

Applications ELISA.
Immunohistochemistry on Frozen Sections.
Reactivities Human

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, AP

Applications ELISA: 1/500-1/1,000.
Western blotting.
Dot- and Slot-Immunoblotting
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation AP

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, FITC

Applications FLISA: 1/200-1/400
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation FITC

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, PE

Applications FLISA: 1 µg/ml.
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation PE

Human IgG (Fc specific) mouse monoclonal antibody, clone MK1A6, Purified

Applications ELISA assay development of total IgG.
Passive hemagglutination assays.
Histological staining of Acetone Fixed Frozen tissue sections.
Reactivities Human

Human IgM mouse monoclonal antibody, clone ICL-931, FITC

Applications ELISA.
Immunohistochemistry on Frozen Sections: 1/40. 
Staining of Human cells and tissue sections.
Reactivities Human
Conjugation FITC

Human IgG mouse monoclonal antibody, clone EFE-565, FITC

Applications ELISA.
Immunohistochemistry on frozen sections: 1/40.
Reactivities Human
Conjugation FITC

Human IgE mouse monoclonal antibody, clone MH25/1, Purified

Applications Immunofluorescence.
Immunohistochemistry on frozen and paraffin sections: 1/5 – 1/10 (Based on the Indirect method, with a normal ABC method the dilution can be 5-10 times higher, but the optimal dilution should be tested by serial dilution.) 
Dilute immediately before use with PBS or TRIS.
Localisation: D ε 1 epitope on Fc Fragment 
Positive control: Polypus nose
Reactivities Human

Human IgA mouse monoclonal antibody, clone BFG-267, FITC

Applications ELISA.
Immunohistochemistry on frozen sections: 1/40.
Reactivities Human
Conjugation FITC

Rat IgM (Mu chain specific) mouse monoclonal antibody, clone HIS40, Supernatant

Applications

Flow cytometry.
Immunofluorescence.
Immunohistochemistry on frozen tissue sections.

Reactivities Rat

Human IgA (Secretory component) mouse monoclonal antibody, clone MC29/12, Purified

Applications Immunohistochemistry on frozen and paraffin sections.
ELISA.
Reactivities Human

Rat IgG2b (heavy chain) mouse monoclonal antibody, clone MARG2b-3, FITC

Applications Flow Cytometry: 5 µg/ml, use 50 µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC

Guinea pig IgG mouse monoclonal antibody, clone MsGp3, Supernatant

Applications ELISA.
Immunohistochemistry on frozen sections.
Reactivities Guinea Pig

Rabbit IgM (B Cell Marker) mouse monoclonal antibody, clone NRBM, Purified

Applications Immunohistochemistry on Frozen Sections.
Flow Cytometry:
Use 10 µl of diluted antibody to label 1x10e6 cells in 100 µl.
Reactivities Rabbit

Human IgA (alpha chain) mouse monoclonal antibody, clone AD3, Purified

Applications ELISA.
Immunohistochemistry on Paraffin Sections: 10 μg/ml.
Positive tissue: colon.
Reactivities Human

Human IgG4 mouse monoclonal antibody, clone MRQ-44, Purified

Applications Immunohistochemistry on Frozen and Formalin-Fixed, Paraffin-Embedded Sections: 1/100-1/500. 
Preparation and Pretreatment:
1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C.
2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water.
3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step.
Positive Control: Tonsil.
Reactivities Human

Human IgA mouse monoclonal antibody, clone DA43D3, Purified

Applications Suitable for Immunohistochemistry on Frozen (1 µg/ml, 1/400) and Paraffin Embedded Sections. Proteinase K pretreatment for antigen retrieval is recommended.
Suggested Positive Control: Human tonsil.
Reactivities Human

Human IgM mouse monoclonal antibody, clone DM3A3, Purified

Applications Immunohistochemistry on frozen sections: 1-2 µg/ml (1:200 - 1:400).
Immunohistochemistry on paraffin sections: Proteinase K pretreatment for antigen retrieval is recommended.
Suggested positive control: Human tonsil.
Reactivities Human

Human IgD (Fc specific) mouse monoclonal antibody, clone LE2, Ig Fraction

Applications Flow cytometry.
Immunohistochemistry on frozen tissue sections.
Reactivities Human