Secondary Antibodies

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Human IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone 4E3, FITC

Applications FLISA:1:200-1:400
Immunofluorescence < / = 1 µg/10e6 cells.
Reactivities Human
Conjugation FITC

Human IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone 4E3, Purified

Applications ELISA: ≤ 5 µg/ml (Ref.1)
Immunofluorescence: ≤ 1 µg/10e6 cells.
Flow Cytometry.
Reactivities Human

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, Biotin

Applications ELISA: 1/5,000-1/20,000.
Immunofluorescence < / = 1 µg/10e6 cells.
Reactivities Human
Conjugation Biotin

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, PE

Applications ELISA.
Immunofluorescence < / = 0.2 µg/10e6 cells.
Reactivities Human
Conjugation PE

Rat IgG Light Chain mouse monoclonal antibody, clone KT100, HRP

Applications ELISA: 1/3000.
Western blot: 1/1000 (DAB as substrate) and 1/10000 (ECL system).
Reactivities Rat
Conjugation HRP

Rat IgG Light Chain mouse monoclonal antibody, clone KT100, HRP

Applications ELISA: 1/3000.
Western blot: 1/1000 (DAB as substrate) and 1/10000 (ECL system).
Reactivities Rat
Conjugation HRP

Rat IgG Light Chain mouse monoclonal antibody, clone KT100, HRP

Applications ELISA: 1/3000.
Western blot: 1/1000 (DAB as substrate) and 1/10000 (ECL system).
Reactivities Rat
Conjugation HRP

Rat IgG Light Chain mouse monoclonal antibody, clone KT100, Aff - Purified

Applications ELISA: 1/3000.
Western Blot: 1/1000 dilution using DAB as substrate, 1/10000 dilution using ECL system.
Reactivities Rat

Rat IgG Light Chain mouse monoclonal antibody, clone KT100, Aff - Purified

Applications ELISA: 1/3000.
Western Blot: 1/1000 dilution using DAB as substrate, 1/10000 dilution using ECL system.
Reactivities Rat

Rat IgG Light Chain mouse monoclonal antibody, clone KT100, Aff - Purified

Applications ELISA: 1/3000.
Western Blot: 1/1000 dilution using DAB as substrate, 1/10000 dilution using ECL system.
Reactivities Rat

Human IgG (Fc specific) mouse monoclonal antibody, clone MK1A6, Purified

Applications ELISA assay development of total IgG.
Passive hemagglutination assays.
Histological staining of Acetone Fixed Frozen tissue sections.
Reactivities Human

Human IgM mouse monoclonal antibody, clone B572M, Purified

Applications ELISA.
Lateral flow assays.
Reactivities Human

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 08C, Purified

Applications ELISA.
Immunoblotting.
Reactivities Chicken

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 14B11, Purified

Applications ELISA.  
Immunoblotting.
Reactivities Chicken

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 9A6, Purified

Applications ELISA.
Immunoblotting.
Reactivities Chicken

Human IgM mouse monoclonal antibody, clone ICL-931, FITC

Applications ELISA.
Immunohistochemistry on Frozen Sections: 1/40. 
Staining of Human cells and tissue sections.
Reactivities Human
Conjugation FITC

Human IgG mouse monoclonal antibody, clone EFE-565, FITC

Applications ELISA.
Immunohistochemistry on frozen sections: 1/40.
Reactivities Human
Conjugation FITC

Human IgE mouse monoclonal antibody, clone MH25/1, Purified

Applications Immunofluorescence.
Immunohistochemistry on frozen and paraffin sections: 1/5 – 1/10 (Based on the Indirect method, with a normal ABC method the dilution can be 5-10 times higher, but the optimal dilution should be tested by serial dilution.) 
Dilute immediately before use with PBS or TRIS.
Localisation: D ε 1 epitope on Fc Fragment 
Positive control: Polypus nose
Reactivities Human

Human IgA mouse monoclonal antibody, clone BFG-267, FITC

Applications ELISA.
Immunohistochemistry on frozen sections: 1/40.
Reactivities Human
Conjugation FITC

Human IgG (Fc specific) mouse monoclonal antibody, clone B1521M, Purified

Applications ELISA.
Reactivities Human

Rat IgM (Mu chain specific) mouse monoclonal antibody, clone HIS40, Supernatant

Applications

Flow cytometry.
Immunofluorescence.
Immunohistochemistry on frozen tissue sections.

Reactivities Rat

Human IgA (Fc specific) mouse monoclonal antibody, clone 15D6, FITC, Purified

Applications ELISA: 1/1000-1/10000. 
Immunofluorescence.
Reactivities Human
Conjugation FITC

Rat IgA mouse monoclonal antibody, clone MARA-2, Purified

Applications ELISA.
Radioimmunoassays.
Reactivities Rat

Human IgA (Secretory component) mouse monoclonal antibody, clone MC29/12, Purified

Applications Immunohistochemistry on frozen and paraffin sections.
ELISA.
Reactivities Human

Rat IgE mouse monoclonal antibody, clone MARE-1, Biotin

Applications ELISA: 5 µg/ml as detecting antibody.
Western Blot.
Reactivities Rat
Conjugation Biotin

Rat IgE mouse monoclonal antibody, clone MARE-1, HRP

Applications ELISA: 0.5 µg/ml.
Western Blot.
Reactivities Rat
Conjugation HRP

Rat IgE mouse monoclonal antibody, clone MARE-1, Purified

Applications ELISA: 5 µg/ml; This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 as detection reagent and purified rat IgE (SA013).
Western Blot.
Reactivities Rat

Rat IgG2a (heavy chain) mouse monoclonal antibody, clone MARG2a-1, FITC

Applications Flow Cytometry: Use 10 µl of 5 µg/ml diluted antibody to label 106 cells in 100 µl.
Reactivities Rat
Conjugation FITC

Rat IgG2b (heavy chain) mouse monoclonal antibody, clone MARG2b-3, FITC

Applications Flow Cytometry: 5 µg/ml, use 50 µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC

Canine IgE mouse monoclonal antibody, clone E6-71A1, Biotin

Applications ELISA: The antibody may be used as a detection antibody in ELISA techniques in combination with SM1498P as capture antibody. 
Western Blotting: Under reducing conditions, this antibody detects a band of approximately 62-65 kDa in samples of affinity purified Dog IgE.
Reactivities Canine, Human
Conjugation Biotin

Guinea pig IgG mouse monoclonal antibody, clone MsGp3, Supernatant

Applications ELISA.
Immunohistochemistry on frozen sections.
Reactivities Guinea Pig

Feline IgA mouse monoclonal antibody, clone IgA9-6A, Purified

Applications Western Blot.
ELISA: This antibody may be used as a capture antibody in an ELISA in combination with either SM1503B or AM01151BT-N as detecting antibody.
Reactivities Feline

Human IgE mouse monoclonal antibody, clone 4C3, Aff - Purified

Applications ELISA: 1 µg/ml.
Sandwich ELISA: 5 µg/ml for coating plates.
Can be used as a Captive antibody for Sandwich ELISA (paired with 1A2 HRP conjugated).
Western Blot: 1 µg/ml.
Reactivities Human

Human IgE mouse monoclonal antibody, clone 4C3, Aff - Purified

Applications ELISA: 1 µg/ml.
Sandwich ELISA: 5 µg/ml for coating plates.
Can be used as a Captive antibody for Sandwich ELISA (paired with 1A2 HRP conjugated).
Western Blot: 1 µg/ml.
Reactivities Human

Human IgE mouse monoclonal antibody, clone 4C3, Aff - Purified

Applications ELISA: 1 µg/ml.
Sandwich ELISA: 5 µg/ml for coating plates.
Can be used as a Captive antibody for Sandwich ELISA (paired with 1A2 HRP conjugated).
Western Blot: 1 µg/ml.
Reactivities Human

Rabbit IgM (B Cell Marker) mouse monoclonal antibody, clone NRBM, Purified

Applications Immunohistochemistry on Frozen Sections.
Flow Cytometry:
Use 10 µl of diluted antibody to label 1x10e6 cells in 100 µl.
Reactivities Rabbit

Human IgA (alpha chain) mouse monoclonal antibody, clone AD3, Purified

Applications ELISA.
Immunohistochemistry on Paraffin Sections: 10 μg/ml.
Positive tissue: colon.
Reactivities Human

USD 300.00

2 Weeks

Human IgE mouse monoclonal antibody, clone 4H10

Reactivities Human

Human IgE (C epsilon 3 specific) (in vitro) mouse monoclonal antibody, clone 4F4cc

Reactivities Human

Human IgG (Fc specific) mouse monoclonal antibody, clone CH1

Reactivities Human

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 52G1

Reactivities Human

Human IgA + IgG + IgM (light chain) mouse monoclonal antibody, clone 7A9

Reactivities Human

Human IgA (secretory) mouse monoclonal antibody, clone B781M, Purified

Applications ELISA: 1/20,000 dilution will yield > 1.0 O.D. using Alkaline Phosphatase conjugated Rabbit anti-Mouse Ig.
Reactivities Human

Human IgE mouse monoclonal antibody, clone 090-11348, Purified

Applications ELISA. 
Recommended antibody pair for Sandwich Immunoassay:
Capture / Detection 
AM00919PU-N / AM00918PU-N
Reactivities Human

Human IgE mouse monoclonal antibody, clone ME.114, Purified

Applications ELISA.
Reactivities Human

Human IgE mouse monoclonal antibody, clone 1A2, HRP

Applications ELISA: 1/500-1/1,000.
Can be used as a Detection antibody in Sandwich ELISA (paired with 4C3).
Reactivities Human
Conjugation HRP

Monkey IgA mouse monoclonal antibody, clone IgA5-3B, Biotin

Applications ELISA: This antibody may be used in a Direct ELISA or as a Detection antibody in a Sandwich ELISA together with product code SM1508P as a Capture reagent.
Reactivities Canine, Feline, Human, Monkey
Conjugation Biotin

Human IgA1 (alpha chain) mouse monoclonal antibody, clone B3506B4, HRP

Applications ELISA: 1/500-1/2000.
Reactivities Human
Conjugation HRP

Human IgD mouse monoclonal antibody, clone IADB6, Purified

Applications ELISA.
Reactivities Human