Secondary Antibodies

View as table Download

Rabbit Monoclonal Anti-Human IgG4 Antibody, Clone RM120

Applications ELISA: 50ng/well - 200ng/well for Capture;, 0.05ug/mL - 0.2ug/mL for Detection;, , WB: 0.5 ug/mL - 2 ug/mL;, ICC: 1 ug/mL-10ug/mL;, IHC: 1 ug/mL-10ug/mL
Reactivities Human IgG4
Conjugation Unconjugated

Rabbit Monoclonal Anti-Mouse IgG1 antibody, clone RM106

Applications ELISA: 0.005ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL, WB: 0.1ug/mL-0.5ug/mL
Reactivities Mouse IgG1
Conjugation Unconjugated

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, Ascites

Applications To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining and immunoblotting of cytoplasmic IgG.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human

Rabbit Monoclonal Anti-Human Gamma Heavy Chain antibody, clone RMG02

Applications ELISA: 0.05ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL
Reactivities Human gamma heavy chain
Conjugation Unconjugated

Mouse IgG1 (heavy chain) rat monoclonal antibody, clone LO-MG1-2, HRP

Applications ELISA: 500 ng/ml. 
This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with SM1491P  as the capture reagent.
Reactivities Mouse
Conjugation HRP

Rabbit Monoclonal Anti-Human Kappa Light Chain antibody, clone RM126

Applications ELISA: 0.05ug/mL-0.2ug/ml; ICC/IHC: 0.5ug/mL-2ug/mL
Reactivities Human kappa light chain
Conjugation Unconjugated

Rabbit Monoclonal Anti-Human Lambda Light Chain antibody, clone RM127

Applications ELISA: 0.05ug/mL-0.2ug/ml; ICC/IHC: 0.5ug/mL-2ug/mL
Reactivities Human lambda light chain
Conjugation Unconjugated

Human IgG (gamma chain specific) mouse monoclonal antibody, clone JDC-10, Biotin

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes: < / = 1 µg/10e6 cells.
ELISA: 1/5,000-1/10,000.
Flow Cytometry: 1 µg / 10e6 cells
Western Blotting
Reactivities Human
Conjugation Biotin