Human IgG (Isotype Specific) Mouse Monoclonal Antibody [Clone ID: NI 335 and NI 343]

CAT#: AM20258AF-N

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, Ascites

Specifications

Product Data

• Clone Name: NI 335 and NI 343
• Applications: ELISA, IF, IP, WB
• Recommended Dilution: To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining and immunoblotting of cytoplasmic IgG.
  General Recommended Dilutions:
  Histochemical Use: 1/50-1/200.
  ELISA: from 1/500 upwards.
  Western blot: from 1/1000 upwards.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly purified IgG isolated from pooled Human serum.
• Specificity: The reactivity of the antiserum is restricted to isotype specific determinants on the Fc part of the IgG molecule. The antiserum reacts with the 4 subclasses of IgG, as tested in indirect binding enzyme immunoassay, immunoblotting and indirect immunoperoxidase staining of cytoplasmic Ig.
  The antiserum does not react with any other component of the human Ig system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
• Formulation: State: Ascites
  State: Lyophilized (Delipidated, heat inactivated) stable ascites without foreign proteins
• Reconstitution Method: Restore by adding 0.5 ml sterile distilled water
• Concentration: 1.0 mg/ml
• Storage: Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
  Avoid Repeated thawing and freezing.