Secondary Antibodies

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Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 7E7, Biotin

Applications Flow Cytometry: ≤ 1 µg/106 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
ELISA: 1/5,000-1/20,000.
Reactivities Rat
Conjugation Biotin

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 7E7, Purified

Applications Flow Cytometry.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
ELISA.
Second step reagent for rat IgG1 Monoclonal Antibodies.
Reactivities Rat

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, Biotin

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
ELISA: Can be used as Capture antibody.
Reactivities Rat
Conjugation Biotin

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, FITC

Applications FLISA: 1/200-1/400.
Flow Cytometry:
  1 ≤ µg/106 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
Reactivities Rat
Conjugation FITC

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, Purified

Applications Flow Cytometry.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
ELISA: Can be used as Capture antibody.
Second step reagent for rat IgG1 Monoclonal Antibodies.
Reactivities Rat

Rat IgG2a (gamma 2a chain specific) mouse monoclonal antibody, clone 2A 8F4, Purified

Applications Flow Cytometry.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
ELISA.
Second step reagent for rat IgG2a Monoclonal Antibodies.
Reactivities Rat

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, Purified

Applications Flow Cytometry.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
ELISA.
Second step reagent for rat IgG2c Monoclonal Antibodies.
Reactivities Rat

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, PE

Applications Flow Cytometry: FITC and BIOT conjugates ≤1 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
ELISA.
Reactivities Rat
Conjugation PE

Rat IgM (Mu chain specific) mouse monoclonal antibody, Biotin

Applications Flow Cytometry (< / = 1.0 µg/10e6 cells).
Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
ELISA.
Second step reagent for rat IgM Monoclonal Antibodies.
Surface IgM Detection.
B Cell Stimulation.
Reactivities Rat
Conjugation Biotin

Rat IgM (Mu chain specific) mouse monoclonal antibody, clone M 2A1, Purified

Applications Flow Cytometry and Immunofluorescence Microscopy:
Identification and enumeration of IgM+ cells.
ELISA.
Second step reagent for rat IgM Monoclonal Antibodies.
Surface IgM Detection.
B Cell Stimulation.

Rabbit IgG mouse monoclonal antibody, clone 2A9, Biotin

Applications ELISA: 1/5,000-1/20,000.
Flow Cytometry.
Reactivities Rabbit
Conjugation Biotin

Chicken IgM (Mu heavy chain specific) mouse monoclonal antibody, clone M-4, Purified

Applications Flow Cytometry: ≤ 1 µg/106 cells.
Indirect Immunofluorescence staining. (Ref.1)
In ovo suppression of Chicken B lymphocytes.
In vitro activation of Chicken peripheral B lymphocytes.
Reactivities Chicken

Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, Biotin

Applications ELISA.
Flow Cytometry: < / = 1 µg/10e6 cells.
Immunofluorescence.
Conjugation Biotin

Human IgM (Mu chain specific) mouse monoclonal antibody, clone UHB, Biotin

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: ≤ 1 µg/10e6 cells.
ELISA: 1/1,000-1/2,000.
Reactivities Human
Conjugation Biotin

Human IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone 4E3, HRP

Applications ELISA: 1/2,000-1/8,000.
Reactivities Human
Conjugation HRP

Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), Biotin

Applications To identify the presence of IgG4 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG4, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/250. 
ELISA: from 1/100 upwards. 
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation Biotin

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 7C2, Biotin

Applications Immunoassays.
Western blot (non-reduced): The typical starting working dilution is 1/10.
Reactivities Chicken, Duck
Conjugation Biotin

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 7C2, Purified

Applications Immunoassays.
Western blot (non-reduced): The typical starting working dilution is 1:10.
Also useful for immunopurification.
Reactivities Chicken, Duck

Human IgG (heavy chain) mouse monoclonal antibody, clone BDI412, Azide Free

Applications ELISA.
Western Blot.
Immunohistochemistry on frozen sections.
Reactivities Human

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, Biotin

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation Biotin

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, FITC

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation FITC

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, PE

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation PE

Mouse IgG (heavy chain) rat monoclonal antibody, clone LO-MG-7, FITC, Purified

Applications Immunoassay.
ELISA: good binding on plastic.
Immunohistochemistry on frozen sections.
Indirect Immunofluorescence.
Reactivities Mouse
Conjugation FITC

Mouse IgA (heavy chain) rat monoclonal antibody, clone LO-MA-7, Purified

Applications Immunoassay.
ELISA: good binding on plastic.
Immunohistochemistry on paraffin sections. 
Reactivities Mouse

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 7C2, Biotin

Reactivities Chicken
Conjugation Biotin

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 7C2, Purified

Applications Immunoassay.
Western Blot  (non-recduced).
The typical starting working dilution is 1/10.
Immunoprecipitation. 
Reactivities Chicken

Human IgM mouse monoclonal antibody, clone ICL-931, Purified

Applications Dot Blot.
ELISA.
Radioimmunoassay.
Immunohistochemistry on frozen sections: 1/40.
Reactivities Human

Human IgG mouse monoclonal antibody, clone EFE-565, HRP, Purified

Applications Dot Blot.
ELISA.
Radioimmunoassay.
Immunohistochemistry on Frozen Sections: 1/40.
Reactivities Human
Conjugation HRP

Human IgG mouse monoclonal antibody, clone EFE-565, Purified

Applications ELISA.
Dot Blot.
Radioimmunoassay.
Immunohistochemistry on frozen sections: 1/40.
Reactivities Human

Human IgA mouse monoclonal antibody, clone BFG-267, HRP, Purified

Applications ELISA: 1/1000.
Radioimmunoassay.
Dot Blot: 1/100.
Immunohistochemistry on frozen sections: 1/40.
Reactivities Human
Conjugation HRP

Human IgA mouse monoclonal antibody, clone BFG-267, Purified

Applications ELISA.
Radioimmunoassay.
Dot Blot.
Immunohistochemistry on frozen sections: 1/40.
Reactivities Human

IGHG1 (heavy chain) mouse monoclonal antibody, clone ICO-97, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT.
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human

IGHG1 (heavy chain) mouse monoclonal antibody, clone ICO-97, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT.
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human

Human IgG (heavy chain) mouse monoclonal antibody, clone B33/20, Purified

Applications ELISA.
Reactivities Human

Rabbit IgG (heavy chain) rat monoclonal antibody, clone RG-1, Aff - Purified

Applications ELISA.
Immunofluorescence.
Reactivities Rabbit

Human IgE mouse monoclonal antibody, clone I27/mAb27

Applications FACS surface, Western Blot, Basophil degranulation, Study of IgE binding to CD23 (Fc epsilon R2). 
Usage recommendation
:
*This monoclonal antibody may be used between 1-10 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Reactivities Human

Human IgG1 (Fc specific) mouse monoclonal antibody, clone 8c/6-39 (HP6091), Purified

Applications ELISA.
Immunodiffusion.
Immunohistochemistry on Frozen Sections.
Not suitable for Paraffin Sections.
Reactivities Human

Human IgG2 (F(ab)2 specific) mouse monoclonal antibody, clone HP6014, Purified

Applications ELISA.
Immunohistochemistry on Frozen Sections.
Reactivities Human

Mouse IgM (heavy chain) rat monoclonal antibody, clone LO-MM-9, FITC

Applications Flow Cytometry: 5 µg/ml; use 5 µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Mouse
Conjugation FITC

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, AP

Applications ELISA: 1/500-1/1,000.
Western blotting.
Dot- and Slot-Immunoblotting
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation AP

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, FITC

Applications FLISA: 1/200-1/400
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation FITC

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, PE

Applications FLISA: 1 µg/ml.
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation PE

Chicken IgG / Chicken IgY (heavy chain) mouse monoclonal antibody, clone 16C7, Purified

Applications Flow Cytometry.
ELISA.
Western Blot under non-reducing conditions.
Reactivities Duck

Rabbit Monoclonal Anti-Mouse IgM (mu-Chain) antibody, clone RM109

Applications ELISA: 0.005ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL
Reactivities Mouse IgM mu-chain
Conjugation Unconjugated

Rabbit Monoclonal Anti-Human IgA1 antibody, clone RM124

Applications ELISA: 50ng/well – 200ng/well (for Capture); 0.05ug/mL – 0.2ug/mL (for Detection); IHC, ICC: 0.5ug/mL – 2ug/mL
Reactivities Human IgA1
Conjugation Unconjugated

Rabbit Monoclonal Anti-Human IgA2 antibody, clone RM125

Applications LISA: 50ng/well – 200ng/well (for Capture); 0.05ug/mL – 0.2ug/mL (for Detection); IHC, ICC: 0.5ug/mL – 2ug/mL
Reactivities Human IgA2
Conjugation Unconjugated

Biotin Conjugated Rabbit Monoclonal Anti-Human Ig Light Chains antibody 3, clone RM129

Applications ELISA: 0.02ug/mL – 0.25ug/mL; IHC, ICC: 0.5ug/mL – 2ug/mL
Reactivities Human Ig light chain
Conjugation Biotin