Chicken IgG / Chicken IgY (light chain) Mouse Monoclonal Antibody [Clone ID: 14A3]

CAT#: SM2274P

Chicken IgG / Chicken IgY (light chain) mouse monoclonal antibody, clone 14A3, Purified

Specifications

Product Data

• Clone Name: 14A3
• Applications: ELISA, FC, WB
• Recommended Dilution: Flow Cytometry.
  ELISA.
  Western Blot.
• Reactivities: Duck
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified Pekin duck yolk IgY. Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.
• Specificity: This antibody recognises the light chain of duck immunoglobulin. Double-staining with clone 14A3 and duck CD8 alpha clone Du CD8-1 (product code MCA2479 ), revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+ /14A3+ cells in duck bursa. The recognition of B cells by 14A3 was confirmed using a recently developed Flag-tagged chicken B cell marker, known as chBAFF-Flag (B cell activating factor). This antibody recognises IgA in Pekin duck bile and can be used as a marker to identify B cells in both Pekin and Muscovy duck and geese studies. This antibody detects a band of approximately 25 kDa in duck cell lysates in Western blotting.
• Formulation: PBS containing 0.09% Sodium Azide
  State: Purified
  State: Liquid purified IgG
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography on Protein G
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.