Chicken IgG / Chicken IgY (light chain) Mouse Monoclonal Antibody [Clone ID: 14A3]

CAT#: SM2274P

Chicken IgG / Chicken IgY (light chain) mouse monoclonal antibody, clone 14A3, Purified


USD 495.00

5 Days*

Size
    • 250 ug

Product Images

Specifications

Product Data
Clone Name 14A3
Applications ELISA, FC, WB
Recommended Dilution Flow Cytometry.
ELISA.
Western Blot.
Reactivities Duck
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Purified Pekin duck yolk IgY. Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.
Specificity This antibody recognises the light chain of duck immunoglobulin. Double-staining with clone 14A3 and duck CD8 alpha clone Du CD8-1 (product code MCA2479 ), revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+ /14A3+ cells in duck bursa. The recognition of B cells by 14A3 was confirmed using a recently developed Flag-tagged chicken B cell marker, known as chBAFF-Flag (B cell activating factor). This antibody recognises IgA in Pekin duck bile and can be used as a marker to identify B cells in both Pekin and Muscovy duck and geese studies. This antibody detects a band of approximately 25 kDa in duck cell lysates in Western blotting.
Formulation PBS containing 0.09% Sodium Azide
State: Purified
State: Liquid purified IgG
Concentration 1.0 mg/ml
Purification Affinity chromatography on Protein G
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.