Secondary Antibodies

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Mouse IgG + IgM (H+L chain), adsorbed goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/100,000
Western Blot: 1/5,000-1/20,000
Immunohistochemistry: 1/2,000-1/10,000
Immunogen Mouse IgG and IgM whole molecules

Mouse IgG + IgM (H+L chain), adsorbed goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy (1:500-1:2,500) and Flow cytometry (1:2,000-1:10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Mouse IgG and IgM whole molecules.

Mouse IgG (H+L chain) goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy (1/500-1/2,500) and Flow cytometry or FACS analysis (1/2,000-1/10,000) as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC
Immunogen Mouse IgG whole molecule.

Mouse IgG (H+L chain) goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy or Immunofluorescence and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Mouse IgG whole molecule.

Rat IgG (H+L chain) goat polyclonal antibody, HRP

Applications Immunoblotting (Western or Dot blot), ELISA, immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/300,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/1,500.
Conjugation HRP
Immunogen Rat IgG whole molecule

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, AP

Applications Suitable for immunoblotting (western or dot blot, 1:500- 1:2,000), ELISA (1:2,000-1:8,000) and Immunohistochemistry (1:200- 1:1,000) as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Conjugation AP
Immunogen Chicken IgG whole molecule.

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Biotin

Applications Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Chicken IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:20,000 to 1:80,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Chicken IgG whole molecule.

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Conjugation FITC
Immunogen Chicken IgG whole molecule.

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Immunogen Chicken IgG whole molecule.

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Chicken IgG whole molecule

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Texas Red

Applications Suitable for Imunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Chicken IgG whole molecule.

Hamster IgG goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 µg of Hamster IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:65,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Hamster IgG whole molecule.

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, FITC

Applications Flow Cytometry: Use 50 µl of 1/25-1/100 diluted antibody to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation FITC
Immunogen Purified Mouse IgG. 
Antisera to Mouse IgG were raised by repeated immunisation of rabbits with highly purified antigen.

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, HRP

Applications ELISA: 1/500 - 1/1000.
Western Blot: 1/2000 - 1/5000.
Immunohistochemistry on frozen, paraffin and resin sections: 1/50.
Reactivities Mouse
Conjugation HRP
Immunogen Purified whole mouse immunoglobulin G

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, Purified

Applications ELISA: 1/500 - 1/1000.
Immunohistochemistry on frozen and paraffin sections: 1/100 - 1/500.
Reactivities Mouse
Immunogen Purified mouse IgG

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, PE

Applications Flow Cytometry: Use 50 µl of 1/20 diluted anti body to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation PE
Immunogen Purified mouse IgG

Mouse IgG (H+L chain), adsorbed goat polyclonal antibody, PE

Applications Flow Cytometry: Use 50 µl of 1/10 diluted antibody to label 10e6 cells in 100 µl.
Reactivities Mouse
Conjugation PE

Mouse IgM (Mu chain specific), adsorbed goat polyclonal antibody, Purified

Applications ELISA: 5µg/ml as coating antibody.
Reactivities Mouse

Rat IgG (H+L chain), Fab Fragment rabbit polyclonal antibody, FITC

Applications Flow Cytometry: 1/200 - 1/400; Use 50 µl of the suggested working dilution to label 10e6 cells in 100 µl.
Reactivities Rat
Conjugation FITC

Rat IgG (H+L chain), Fab Fragment rabbit polyclonal antibody, HRP

Applications ELISA: 1/1000 - 1/2000.
Immunohistochemistry on frozen sections: 1/50.
Immunohistochemistry on paraffin sections: 1/50.
Immunohistochemistry on resin sections: 1/50.
Reactivities Rat
Conjugation HRP
Immunogen Purified rat immunoglobulin G

Rat IgG (H+L chain), Fab Fragment rabbit polyclonal antibody, Purified

Applications ELISA: 1/250 - 1/1000.
Immunodiffusion.
Reactivities Rat
Immunogen Purified polyclonal rat IgG

Rat IgG (H+L chain), F(ab)2 Fragment, adsorbed goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/25 - 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC
Immunogen Rat IgG

Rat IgG (H+L chain), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Flow cytometry (1/5 - 1/10): use 50 µl of the suggested working dilution to label 10e6 cells in 100 µl.
Reactivities Rat
Conjugation PE
Immunogen Rat IgG

Human IgA + IgG + IgM goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of 0.1-0.5 µg/ml diluted antibody to label 106 cells in 100 µl.
Reactivities Human
Conjugation FITC
Immunogen Polyclonal Human Ig.

Hamster IgG (adsorbed) goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/50; Use 50µl of the suggested working dilution to label 1 x 10e6 cells in 100µl.
Reactivities Hamster
Conjugation FITC
Immunogen Hamster IgG

Mouse IgG (H+L chain), adsorbed goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 50µl of 1/50-1/200 diluted antibody to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation FITC
Immunogen Mouse IgG

Bovine IgM sheep polyclonal antibody, HRP

Applications ELISA: 1/10,000 - 1/100,000.
Reactivities Bovine
Conjugation HRP
Immunogen Purified bovine IgM

Bovine IgG1 sheep polyclonal antibody, HRP

Applications ELISA: 1/10000-1/100000.
Reactivities Bovine
Conjugation HRP
Immunogen Purified Bovine IgG1

Canine IgM goat polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Reactivities Canine
Conjugation AP
Immunogen Purified Canine IgM.

Bovine IgG2 sheep polyclonal antibody, HRP

Applications ELISA: 1:10,000-1:100,000.
Reactivities Bovine
Conjugation HRP
Immunogen Purified bovine IgG2

Equine IgA goat polyclonal antibody, HRP

Applications ELISA: 1/10,000 - 1/100,000.
Reactivities Equine
Conjugation HRP
Immunogen Purified equine IgA.

Equine IgG goat polyclonal antibody, HRP

Applications ELISA: 1/10,000 - 1/100,000.
Reactivities Equine
Conjugation HRP
Immunogen Purified equine IgG (T).

Equine IgM goat polyclonal antibody, HRP

Applications ELISA: 1/10,000 - 1/100,000.
Reactivities Equine
Conjugation HRP
Immunogen Purified equine IgM

Feline IgA goat polyclonal antibody, HRP

Applications ELISA: 1/10,000 - 1/100,000.
Reactivities Feline
Conjugation HRP
Immunogen Purified feline IgA.

Feline IgG (Fc specific) goat polyclonal antibody, HRP

Applications ELISA: 1/10,000.
Reactivities Feline
Conjugation HRP
Immunogen Purified Feline IgG Fc fragment.

Porcine IgG F(c) specific goat polyclonal antibody, HRP

Applications ELISA: 1/10,000-1/100,000.
Reactivities Porcine
Conjugation HRP
Immunogen Purified Porcine IgG.

Chicken IgM goat polyclonal antibody, HRP

Applications ELISA (1/10,000-1/100,000).
Reactivities Chicken
Conjugation HRP
Immunogen Purified chicken IgM.

Chicken IgA goat polyclonal antibody, HRP

Applications ELISA (1/10,000-1/100,000).
Reactivities Chicken
Conjugation HRP
Immunogen Purified chicken IgA.

Chicken IgG / Chicken IgY (Fc specific) goat polyclonal antibody, HRP

Applications ELISA: 1/10,000-1/100,000.
Reactivities Chicken
Conjugation HRP
Immunogen Purified Chicken IgG (Fc) fragment.

Rabbit IgG (H+L chain) sheep polyclonal antibody, FITC

Applications Flow Cyometry: 1/25 - 1/50; Use 50µl of the suggested working dilution to label 10e6 cells in 100µl.
Immunohistochemistry on frozen sections: 1/25 - 1/50.
Conjugation FITC
Immunogen Rabbit IgG

Porcine IgG (H+L chain) goat polyclonal antibody, HRP

Applications ELISA: 1:10,000 - 1:100,000.
Reactivities Porcine
Conjugation HRP

Porcine IgG (H+L chain) goat polyclonal antibody, Aff - Purified

Applications ELISA: 1:100 - 1:10,000.
Reactivities Porcine

Canine IgE goat polyclonal antibody, FITC

Applications Flow Cytometry: 1:200 - 1:2,000.
Reactivities Canine
Conjugation FITC

Canine IgG1 goat polyclonal antibody, FITC

Applications Flow Cytometry: 1:200 - 1:2000.
Reactivities Canine
Conjugation FITC

Sheep IgA rabbit polyclonal antibody, Purified

Applications ELISA: 1:100 - 1:10,000.
Reactivities Sheep

Sheep IgM rabbit polyclonal antibody, FITC

Applications Flow Cytometry: 1:200 - 1:2000.
Reactivities Sheep
Conjugation FITC

Sheep IgM rabbit polyclonal antibody, HRP

Applications ELISA: 1/10,000-1/100,000.
Reactivities Sheep
Conjugation HRP

Sheep IgM rabbit polyclonal antibody, Purified

Applications ELISA: 1:100 - 1:10,000.
Reactivities Sheep

Mouse lambda light chain goat polyclonal antibody, AP

Applications ELISA (1/1,000-1/2,000).
Reactivities Mouse
Conjugation AP
Immunogen Mouse paraproteins with lambda 1 and lambda 2 light chains

Mouse lambda light chain goat polyclonal antibody, Biotin

Applications ELISA: 1/1000 - 1/2000.
Reactivities Mouse
Conjugation Biotin
Immunogen Mouse paraproteins with lambda 1 and lambda 2 light chains