Mouse IgG (H+L chain), F(ab)2 Fragment Rabbit Polyclonal Antibody

CAT#: SP1012F

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, FITC

Specifications

Product Data

• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 50 µl of 1/25-1/100 diluted antibody to label 10⁶ cells in 100 µl.
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified Mouse IgG.
  Antisera to Mouse IgG were raised by repeated immunisation of rabbits with highly purified antigen.
• Specificity: Mouse IgG (all subclasses). Cross reactivity with mouse IgM and IgA is expected. Cross reactivity with rat IgG is expected. Cross reactivity with human serum proteins has been minimised by solid phase adsorption.
• Formulation: PBS, pH 7.2
  Label: FITC
  State: Liquid F(ab')2 fragment of IgG
  Preservative: 0.09% Sodium Azide
  Label: Fluorescein Isothiocyanate Isomer I
• Concentration: 1.0 mg/ml
• Purification: Purified IgG was prepared from whole serum by affinity chromatography. F(ab')₂ fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove the remaining intact IgG or Fc fragments.
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.