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GCA (Myc-DDK-tagged)-Human grancalcin, EF-hand calcium binding protein (GCA)

Vector pCMV6-Entry
Tag Myc-DDK
Mammalian Cell Selection Neomycin

GCA (GFP-tagged) - Human grancalcin, EF-hand calcium binding protein (GCA)

Vector pCMV6-AC-GFP
Tag TurboGFP
Mammalian Cell Selection Neomycin
  • TrueORF®

GCA - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.

Format 2 gRNA vectors, 1 linear donor
Donor DNA EF1a-GFP-P2A-Puro
KN402677 is the updated version of KN202677.

Lenti ORF clone of Human grancalcin, EF-hand calcium binding protein (GCA), Myc-DDK-tagged

Vector pLenti-C-Myc-DDK-P2A-Puro
Tag Myc-DDK
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF clone of Human grancalcin, EF-hand calcium binding protein (GCA), mGFP tagged

Vector pLenti-C-mGFP-P2A-Puro
Tag mGFP
Mammalian Cell Selection Puromycin
  • LentiORF®

GCA (untagged)-Human grancalcin, EF-hand calcium binding protein (GCA)

Vector pCMV6-XL5
Tag Tag Free
Mammalian Cell Selection None

GCA (untagged)-Human grancalcin, EF-hand calcium binding protein (GCA)

Vector pCMV6-AC
Tag Tag Free
Mammalian Cell Selection Neomycin

GCA - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Format Lentiviral particles
Vector pGFP-C-shLenti

Grancalcin (1-217, His-tag) human recombinant protein, 0.25 mg

Tag His-tag
Expression Host E. coli

Grancalcin (1-217, His-tag) human recombinant protein, 50 µg

Tag His-tag
Expression Host E. coli

GCA CRISPRa kit - CRISPR gene activation of human grancalcin

Format 3gRNAs, 1 scramble ctrl and 1 enhancer vector

qPCR primer pairs and template standards against Homo sapiens gene GCA

Application Plasmid of exact quantity for transcript copy number calculation

qSTAR qPCR primer pairs against Homo sapiens gene GCA

GCA HEK293T cell transient overexpression lysate (as WB positive control)

Tag C-Myc/DDK
Expression Host HEK293T
Applications WB

Transient overexpression lysate of grancalcin, EF-hand calcium binding protein (GCA)

Tag C-Myc/DDK
Expression Host HEK293T
Applications WB

GCA MS Standard C13 and N15-labeled recombinant protein (NP_036330)

Tag C-Myc/DDK
Expression Host HEK293

Transient overexpression of GCA (NM_012198) in HEK293T cells paraffin embedded controls for ICC/IHC staining

GCA - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector

Format Lentiviral plasmids
Vector pGFP-C-shLenti
E. coli Selection Chloramphenicol
Mammalian Cell Selection Puromycin

Recombinant protein of human grancalcin, EF-hand calcium binding protein (GCA)

Tag N-GST
Expression Host E. coli

Recombinant protein of human grancalcin, EF-hand calcium binding protein (GCA)

Tag N-GST
Expression Host E. coli

Recombinant protein of human grancalcin, EF-hand calcium binding protein (GCA)

Tag N-GST
Expression Host E. coli

Recombinant protein of human grancalcin, EF-hand calcium binding protein (GCA)

Tag N-GST
Expression Host E. coli

Recombinant protein of human grancalcin, EF-hand calcium binding protein (GCA)

Tag tag free
Expression Host E. coli

Recombinant protein of human grancalcin, EF-hand calcium binding protein (GCA)

Tag tag free
Expression Host E. coli

Recombinant protein of human grancalcin, EF-hand calcium binding protein (GCA)

Tag tag free
Expression Host E. coli

GCA - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector

Format Retroviral plasmids
Vector pRS

Transient overexpression of GCA (NM_012198) in HEK293T cells paraffin embedded 4 um sections, controls for ICC/IHC staining, 5 slides per pack

Transient overexpression of GCA (NM_012198) in HEK293T cells paraffin embedded 4 um sections, controls for ICC/IHC staining, 25 slides per pack