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Nans (Myc-DDK-tagged) - Mouse N-acetylneuraminic acid synthase (sialic acid synthase) (Nans)

Vector pCMV6-Entry
Tag Myc-DDK
Mammalian Cell Selection Neomycin
  • TrueORF®

Nans - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.

Format 2 gRNA vectors, 1 linear donor
Donor DNA EF1a-GFP-P2A-Puro
KN510724 is the updated version of KN310724.

Nans (GFP-tagged) - Mouse N-acetylneuraminic acid synthase (sialic acid synthase) (Nans)

Vector pCMV6-AC-GFP
Tag TurboGFP
Mammalian Cell Selection Neomycin
  • TrueORF®

Lenti ORF clone of Nans (Myc-DDK-tagged) - Mouse N-acetylneuraminic acid synthase (sialic acid synthase) (Nans)

Vector pLenti-C-Myc-DDK-P2A-Puro
Tag Myc-DDK
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF particles, Nans (Myc-DDK-tagged) - Mouse N-acetylneuraminic acid synthase (sialic acid synthase) (Nans), 200ul, >10^7 TU/mL

Vector pLenti-C-Myc-DDK-P2A-Puro
Tag Myc-DDK
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF clone of Nans (mGFP-tagged) - Mouse N-acetylneuraminic acid synthase (sialic acid synthase) (Nans)

Vector pLenti-C-mGFP-P2A-Puro
Tag mGFP
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF particles, Nans (GFP-tagged) - Mouse N-acetylneuraminic acid synthase (sialic acid synthase) (Nans), 200ul, >10^7 TU/mL

Vector pLenti-C-mGFP-P2A-Puro
Tag mGFP
Mammalian Cell Selection Puromycin
  • LentiORF®

Nans (untagged) - Mouse N-acetylneuraminic acid synthase (sialic acid synthase) (Nans), (10ug)

Vector PCMV6-Kan/Neo
Tag Tag Free
Mammalian Cell Selection Neomycin

qPCR primer pairs and template standards against Mus musculus gene Nans

Application Plasmid of exact quantity for transcript copy number calculation

qSTAR qPCR primer pairs against Mus musculus gene Nans

Nans (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).

Nans - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector

Format Lentiviral plasmids
Vector pGFP-C-shLenti

Nans - Mouse shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Format Lentiviral particles
Vector pGFP-C-shLenti

Purified recombinant protein of Mouse N-acetylneuraminic acid synthase (sialic acid synthase) (Nans), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Tag C-MYC/DDK
Expression Host HEK293T

Nans - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

Format Retroviral plasmids
Vector pRS