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Rngtt - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.

Format 2 gRNA vectors, 1 linear donor
Donor DNA EF1a-GFP-P2A-Puro
KN514964 is the updated version of KN314964.

Rngtt (GFP-tagged) - Mouse RNA guanylyltransferase and 5'-phosphatase (Rngtt)

Vector pCMV6-AC-GFP
Tag TurboGFP
Mammalian Cell Selection Neomycin
  • TrueORF®

Rngtt (Myc-DDK-tagged) - Mouse RNA guanylyltransferase and 5'-phosphatase (Rngtt)

Vector pCMV6-Entry
Tag Myc-DDK
Mammalian Cell Selection Neomycin
  • TrueORF®

Lenti ORF clone of Rngtt (Myc-DDK-tagged) - Mouse RNA guanylyltransferase and 5'-phosphatase (Rngtt)

Vector pLenti-C-Myc-DDK-P2A-Puro
Tag Myc-DDK
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF particles, Rngtt (Myc-DDK-tagged) - Mouse RNA guanylyltransferase and 5'-phosphatase (Rngtt), 200ul, >10^7 TU/mL

Vector pLenti-C-Myc-DDK-P2A-Puro
Tag Myc-DDK
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF clone of Rngtt (mGFP-tagged) - Mouse RNA guanylyltransferase and 5'-phosphatase (Rngtt)

Vector pLenti-C-mGFP-P2A-Puro
Tag mGFP
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF particles, Rngtt (GFP-tagged) - Mouse RNA guanylyltransferase and 5'-phosphatase (Rngtt), 200ul, >10^7 TU/mL

Vector pLenti-C-mGFP-P2A-Puro
Tag mGFP
Mammalian Cell Selection Puromycin
  • LentiORF®

Rngtt (untagged) - Mouse RNA guanylyltransferase and 5'-phosphatase (Rngtt), (10ug)

Vector PCMV6-Kan/Neo
Tag Tag Free
Mammalian Cell Selection Neomycin

Rngtt CRISPRa kit - CRISPR gene activation of mouse RNA guanylyltransferase and 5'-phosphatase

Format 3gRNAs, 1 scramble ctrl and 1 enhancer vector

qPCR primer pairs and template standards against Mus musculus gene Rngtt

Application Plasmid of exact quantity for transcript copy number calculation

qSTAR qPCR primer pairs against Mus musculus gene Rngtt

Rngtt (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).

Rngtt - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector

Format Lentiviral plasmids
Vector pGFP-C-shLenti

Rngtt - Mouse shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Format Lentiviral particles
Vector pGFP-C-shLenti

Rngtt - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

Format Retroviral plasmids
Vector pRS