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Taf1 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.

Format 2 gRNA vectors, 1 linear donor
Donor DNA EF1a-GFP-P2A-Puro
KN517135 is the updated version of KN317135.

Taf1 (GFP-tagged) - Mouse TAF1 RNA polymerase II TATA box binding protein (TBP)-associated factor (Taf1), (10ug)

Vector pCMV6-AC-GFP
Tag TurboGFP
Mammalian Cell Selection Neomycin
  • TrueORF®

Taf1 (Myc-DDK-tagged) - Mouse TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (Taf1)

Vector pCMV6-Entry
Tag Myc-DDK
Mammalian Cell Selection Neomycin
  • TrueORF®

Taf1 (myc-DDK-tagged) - Mouse TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (Taf1)

Vector pCMV6-Entry
Tag Myc-DDK
Mammalian Cell Selection Neomycin
  • TrueORF®

Taf1 CRISPRa kit - CRISPR gene activation of mouse TATA-box binding protein associated factor 1

Format 3gRNAs, 1 scramble ctrl and 1 enhancer vector

Taf1 (untagged) - Mouse TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (Taf1), (10ug)

Vector pCMV6-Entry
Tag Tag Free
Mammalian Cell Selection Neomycin

Taf1 (untagged) - Mouse TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (Taf1)

Vector pCMV6-Entry
Tag Tag Free
Mammalian Cell Selection Neomycin

qSTAR qPCR primer pairs against Mus musculus gene Taf1

Taf1 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).

Taf1 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector

Format Lentiviral plasmids
Vector pGFP-C-shLenti

Taf1 - Mouse shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Format Lentiviral particles
Vector pGFP-C-shLenti

Taf1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

Format Retroviral plasmids
Vector pRS