AAV Serotyping Kit

The Importance of AAV Serotyping

Each AAV serotype exhibits unique characteristics, including tissue tropism and transduction efficiency, making serotype selection a critical factor in the success of gene delivery. When handling a new cell type or tissue where the optimal serotype is unknown, serotyping kits provide a solution, allowing you to test each serotype's transduction efficiency before starting your experiment.

OriGene offers AAV serotyping kits that enable researchers to select the most appropriate serotype for their specific research or therapeutic applications.

Key Features and Benefits

  • Ultra-Purified Particles: The kit includes ultra-purified AAV viral particles, ensuring high-quality and consistent particles for gene delivery both in vitro & in vivo.
  • Guaranteed High Titers: OriGene guarantees viral titers of >1013 GC/mL, providing researchers with ample material for efficient gene transduction.
  • GFP Tagged: The GFP tag in each particles allows for easy visualization of transduction.
AAV
Figure 1: In vitro transduction efficiency of HEK293T cell line with different AAV serotypes. This image shows that AAV DJ is more efficient than AAV 2 for gene delivery in HEK293T cells.
Name Description Catalog #
AAV Serotyping Kit I This kit contains pre-made AAV-2, AAV-5, AAV-6, AAV-8, and AAV-9 control particles for testing each serotype's infection efficiency in your desired cell type. CV500001
AAV Serotyping Kit II This kit contains pre-made AAV-1, AAV-3, AAV-7, AAV-rh.10, AAV-DJ, and AAV-PHP.eB control particles for testing each serotypes infection efficiency in your desired cell type. CV500002

AAV Serotype Selection Guide

Different AAV serotypes exhibit varying transduction efficiencies across different cell types. The table below provides the selection guide for in vitro and in vivo applications1.

Note: The numbers in the table are normalized to the infectivity rate of AAV-2. AAV-2 = 100. ND = Not Determined

Cell Line AAV-1 AAV-2 AAV-3 AAV-4 AAV-5 AAV-6 AAV-8 AAV-9 AAV-DJ AAV-DJ/8
Huh-7 13 100 2.5 0.0 0.1 10 0.7 0.0 500 0.2
HEK293 25 100 2.5 0.1 0.1 5 0.7 0.1 500 0.3
HeLa 3 100 2.0 0.1 6.7 1 0.2 0.1 667 0.2
HepG2 3 100 16.7 0.3 1.7 5 0.3 ND 1250 0.5
Hep1A 20 100 0.2 1.0 0.1 1 0.2 0.0 400 0.1
911 17 100 11 0.2 0.1 17 0.1 ND 500 0.0
CHO 100 100 14 1.4 333 50 10 1.0 25000 5.0
COS 33 100 33 3.3 5.0 14 2.0 0.5 500 0.3
MeWo 10 100 20 0.3 6.7 10 1.0 0.2 2857 1.0
NIH3T3 10 100 2.9 2.9 0.3 10 0.3 ND 500 0.1
A549 14 100 20 ND 0.5 10 0.5 0.1 1000 0.1
HT1180 20 100 10 0.1 0.3 33 0.5 0.1 333 0.2
Monocytes 1111 100 ND ND 125 1429 ND ND 100 ND
Immature DC 2500 100 ND ND 222 2857 ND ND 200 ND
Mature DC 2222 100 ND ND 333 3333 ND ND 100 ND

1. Grimm D, Lee JS, Wang L, et al. In vitro and in vivo gene therapy vector evolution via multispecies interbreeding and retargeting of adeno-associated viruses. J Virol. 2008;82(12):5887-5911. doi:10.1128/JVI.00254-08

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