Myeloperoxidase (MPO) Mouse Monoclonal Antibody [Clone ID: OTI4B1]

CAT#: CF814075

Carrier-free (BSA/glycerol-free) MPO mouse monoclonal antibody, clone OTI4B1

Specifications

Product Data

• Clone Name: OTI4B1
• Applications: ELISA
• Recommended Dilution: ELISA 1:5000-10000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 155-745 of human MPO (NP_000241) produced in 293F cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Predicted Protein Size: 83.7 kDa
• Gene Name: Homo sapiens myeloperoxidase (MPO), mRNA.
• Database Link:
  NP_000241
  Entrez Gene 4353 Human
• Background: Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]

Reference Data

• Protein Families: Druggable Genome