GFAP Mouse Monoclonal Antibody [Clone ID: OTI3A12]

CAT#: CF814384

Carrier-free (BSA/glycerol-free) GFAP mouse monoclonal antibody, clone OTI3A12

Specifications

Product Data

• Clone Name: OTI3A12
• Applications: ELISA
• Recommended Dilution: ELISA 1:5000-10000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 292-432 of human GFAP (NP_002046) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Predicted Protein Size: 49.9 kDa
• Gene Name: Homo sapiens glial fibrillary acidic protein (GFAP), transcript variant 1, mRNA.
• Database Link:
  NP_002046
  Entrez Gene 2670 Human
• Background: This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
• Synonyms: ALXDRD

Reference Data

• Protein Families: ES Cell Differentiation/IPS