Serotonin transporter (SLC6A4) (579-599) Rabbit Polyclonal Antibody

CAT#: 24330

Serotonin transporter (SLC6A4) (579-599) rabbit polyclonal antibody

Product Images

Staining with 5-HT (Serotonin) Transporter Antibody 24330

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: IHC Quality Control: The ImmunoStar Serotonin Transporter (5HTT) was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat raphe nuclei, hypothalamus, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Western blot analysis using rat brain extracts of cortex, hypothalamus, midbrain, and hindbrain, specifically labels a single band. Immunolabeling is completely abolished by preadsorption with synthetic rat 5HT transporter (602–622).
  Tissue: Rat raphe nuclei, hypothalamus, cortex, and spinal cord.
  Perfusion Fixation
  • Fixation: 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4; 500 mL over 20 min.
  • Post Fixation: 1.5 hour at 4°C in 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4.
  • Note: If needed, low levels of glutaraldehyde (0.1–0.3%) may be used in conjunction with paraformaldehyde.
  
  Sections: 10 µm cryostat or 50 µm vibratome.
  Tissue Incubation: 18–24 hours at 2–8°C
  Detection System: Use Cy3 or Bn/Av-HRP reagents at dilutions recommended by the manufacturers.
  Suggested Dilution:
  1/800 – 1/1,000 in PBS/0.3% Triton X-100 – indirect immunofluorescence.
  1/10,000 – 1/15,000 in PBS/0.3% Triton X-100 – Bn/Av-HRP immunohistochemistry.
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide sequence corresponding to amino acids (602–622) of rat 5HT transporter coupled to keyhole limpet hemocyanin (KLH).
• Specificity: Reacts with: Human, Marine, Monkey, Mouse, Rat, Sparrow.
  Predicted to React With: Bird, Bovine, Frog, Guinea Pig, Monkey, Sheep.
• Formulation: State: Serum
  State: Lyophilized whole serum
  Preservative: ≤ 0.09% sodium azide
• Conjugation: Unconjugated
• Storage: Do not reconstitute until ready to use since the product is most stable when lyophilized. The product does not need to be kept cooled during shipping; however, for long-term storage, store lyophilized antibody until ready to use at -15ºC or lower. Reconstitute with 100 μL of distilled or deionized water. After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. To avoid freeze/thaw cycles, dilute unused antibody with PBS or Tris buffer at a dilution no higher than 1/10, then aliquot and freeze at -15ºC or lower.
  Refer to the Instruction Manual available online at www.immunostar.com for information on tissue preparation, immunostaining techniques, troubleshooting, and formulas.
• Gene Name: Homo sapiens solute carrier family 6 member 4 (SLC6A4)
• Database Link:
  Entrez Gene 15567 MouseEntrez Gene 25553 RatEntrez Gene 6532 Human
  UniProt ID P31645
• Synonyms: 5HT transporter, 5HTT, HTT, Sodium-dependent serotonin transporter

Reference Data

• Protein Families: Druggable Genome, Transmembrane