Amyloid beta (1-42 specific) Mouse Monoclonal Antibody [Clone ID: 8G7]

CAT#: AM00003PU-N

Amyloid beta (1-42 specific) mouse monoclonal antibody, clone 8G7, Purified

Product Images

Figure 1. Immunoblot Analysis Amyloid beta A4 peptides: Lane 1: bA4 (1-40), Lane 2: bA4 (1-42), Lane 3: bA4 (1-43) were applied on SDS-PAGE and transferred to a PVDF membrane. The Immunoblot was probed with 2 g/ml mab bA4 (42)-8G7for 1h at 15-22C and develo

Specifications

Product Data

• Clone Name: 8G7
• Applications: ELISA, IF, WB
• Recommended Dilution: ELISA: 0.05 µg/ml.
  Immunoblotting: 1.0 µg/ml for HRPO/ECL detection.
  Recommended buffer: Casein/Tween20 based blocking and blot incubation buffer.
  Immunocytochemistry: 0.1-1 µg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: C-terminal peptide (aa 1-42) of Amyloid beta A4 - conjugated to KLH
• Specificity: This antibody specifically interacts with the C-Terminus of beta-Amyloid (1-42) and does not crossreact with beta-Amyloid (1-40).
• Formulation: 1ml 2 x PBS/0.09% Sodium Azide/PEG and Sucrose
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with 1.0 ml H2O (15 min, RT).
• Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
• Background: The beta-amyloid peptide (beta A4), proteolytically released from the amyloid precursor protein (APP), is the principal component of senile plaques in Alzheimer's disease. Cleavage of APP by alpha-secretase or alternatively by beta-secretase leads to generation and extracellular release of soluble APP peptides, S-APP-alpha and S-APP-beta, respectively, and the retention of corresponding membrane-anchored C-terminal fragments, C83 and C99. Subsequent processing of C83 by gamma-secretase yields P3 peptides. This is the major secretory pathway and is nonamyloidogenic. Alternatively, presenilin/nicastrin-mediated gamma-secretase processing of C99 releases the amyloid beta proteins, amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42), major components of amyloid plaques, and the cytotoxic C-terminal fragments, gamma-CTF(50), gamma-CTF(57) and gamma-CTF(59).