alpha 1 Catenin (CTNNA1) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 15D9]

CAT#: AM00014PU-N

alpha 1 Catenin (CTNNA1) (incl. pos. control) mouse monoclonal antibody, clone 15D9, Purified

Product Images

Detection of endogenous alpha catenin: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab a-Cat-15D9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2

Specifications

Product Data

• Clone Name: 15D9
• Applications: ELISA, IF, IP, WB
• Recommended Dilution: Western Blot: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  Included Positive Control: Cell lysate from untreated A431 cells.
  ELISA: 0.05 µg/ml.
  Immunoprecipitation: 1-10 µg per 10e6 vanadate-treated A431 cells.
  Immunocytochemistry: 0.1-1 µg/ml. Mab Anti-Catenin Clone 15D9 may tolerate formaldehyd.
• Reactivities: Canine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant α-Catenin
• Specificity: This antibody specifically recognizes α-Catenin in Western blot at 102 kDa.
• Formulation: 1 ml 2x PBS / 0.09% Sodium Azide / PEG and Sucrose
  State: Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore with 1 ml H2O (15 min, RT).
• Purification: Size Exclusion Chromatography
• Storage: Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 1495 Human
• Background: The α-, β- and γ-catenins are cytoplasmic proteins mediating the interaction of Ca2+-dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. The N-terminus of α-catenin directly interacts with exon 3 of β-catenin. The C-terminus of α-catenin interacts with the actin filament network.
• Synonyms: Alpha E-catenin, Alpha Catenin