beta Catenin (CTNNB1) (exon 3) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9G2]

CAT#: AM00020BT-N

beta Catenin (CTNNB1) (exon 3) (incl. pos. control) mouse monoclonal antibody, clone 9G2, Biotin

Specifications

Product Data

• Clone Name: 9G2
• Applications: ELISA, IF, IHC, IP, WB
• Recommended Dilution: ELISA: 0.05 μg/ml.
  Western Blot: 0.5 μg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  Immunoprecipitation: 1-10 μg per 10⁶ vanadate treated A431 cells.
  Immunocytochemistry: 0.1-1 μg/ml.
  Immunohistochemistry on Paraffin Sections.
  Included Positive Control: Cell lysate from untreated SW480 cells (for details see "Protocols")
• Reactivities: Canine, Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant β-Catenin.
  Epitope: Exon 3/Alpha-Catenin binding site
• Specificity: This antibody specifically interacts with exon 3 (alpha-Catenin-binding site) of beta-Catenin.
• Formulation: PBS containing 0.09% Sodium Azide, PEG and Sucrose
  Label: Biotin
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with 1 ml H2O (15 mn at RT)
• Purification: Size Exclusion Chromatography
• Conjugation: Biotin
• Storage: Store the antibody (aliquote in liquid nitrogen) at -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 90 kDa
• Database Link:
  Entrez Gene 1499 Human
• Background: The α-, β- and γ-catenins are cytoplasmic proteins mediating the interaction of Ca2+-dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. The direct interaction of β-catenin with the cytoplasmic domain of cadherins plays a crucial role for cell-cell adhesion and signal transmission between neighbouring cells. Recent studies indicate that β-catenin may also play a role in tumorigenesis since it forms complexes with the tumor suppressor gene product APC. β-catenin directly interacts and constitutively activates transcription factors of the TCF/LEF gene family. Thus it is proposed that β-catenin plays a dual role not only in the maintainance and regulation of cell-cell interactions but also in the regulation of gene activity.
• Synonyms: CTNNB1, CTNNB, Beta-catenin
• Note: Protocol: Positive Control: Cell lysate from untreated SW480 cells.
  Formulation: Lyophilized Cell Lysate from Serum starved SW480 cells.
  
  Stability:
  Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
  
  Application:
  The Positive Control Cell Lysate is recommended for immunoblot applications. 20 µl of Positive Control cell lysate correspond to ca. 20.000 cells.
  Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins.
  
  Note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as Immunoprecipitation.
  
  Storage:
  Aliquote and store frozen.
  Avoid repeated freeze/thaw cycles.
  Shelf life: one year from despatch.