EGFR pThr678 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 3F2]

CAT#: AM00029BT-N

EGFR pThr678 (incl. pos. control) mouse monoclonal antibody, clone 3F2, Biotin


USD 530.00

2 Weeks*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name 3F2
Applications ELISA, IP, WB
Recommended Dilution ELISA: 0.05 μg/ml.
Western Blot: 0.5 μg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Immunoprecipitation: 1 - 10 µg per 106 vanadate treated A431 cells.
Luminex.
Reactivities Canine, Human, Mouse, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Phosphopeptide conjugated to KLH
Specificity This antibody specifically recognizes EGFR phosphorylated at Threonine 678.
Formulation PBS/0.09% Na-Azide/PEG and Sucrose
Label: Biotin
State: Liquid
Concentration lot specific
Purification Size exclusion chromatography
Conjugation Biotin
Storage Store the antibody (aliquote in liquid nitrogen) at -80°C.
Avoid repeated freezing and thawing.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Stability Shelf life: one year from despatch.
Background EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases.
Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity; phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.
Synonyms Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.