EGFR pTyr869 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 12A3]

CAT#: AM00031BT-N

EGFR pTyr869 (incl. pos. control) mouse monoclonal antibody, clone 12A3, Biotin

Specifications

Product Data

• Clone Name: 12A3
• Applications: ELISA, IF, IHC, IP, WB
• Recommended Dilution: Western Blot: 1 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  ELISA: 0.1 µg/ml (protein ELISA).
  Immunoprecipitation: 1 - 10 μg per 106 vanadate treated A431 cells.
  Immunocytochemistry: 1 - 10 μg/ml.
  Immunohistochemistry on frozen and paraffin sections.
  Luminex.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Phosphopeptide conjugated to KLH.
• Specificity: This antibody specifically recognizes EGFR phosphorylated at Tyrosine 869 and detects EGFR activation after interaction with src kinases. Mab 12A3 does not crossreact with the highly homologues pTyr 877 of activated erbB2.
• Formulation: PBS/0.09% Na-Azide/PEG and Sucrose
  Label: Biotin
  State: Liquid
• Concentration: lot specific
• Purification: Size exclusion chromatography
• Conjugation: Biotin
• Storage: Store the antibody (aliquote in liquid nitrogen) at -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 1956 Human
• Background: EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases.
  Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity; phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.
• Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1