EGFR pTyr1173 Mouse Monoclonal Antibody [Clone ID: 9H2]

CAT#: AM00042FC-N

EGFR pTyr1173 mouse monoclonal antibody, clone 9H2, FITC

Specifications

Product Data

• Clone Name: 9H2
• Applications: IF
• Recommended Dilution: Immunocytochemistry: Use at 1-10 μg/ml.
  For ELISA, Immunoprecipitation and Immunoblotting, use Purified antibody (Cat.-No. AM00042PU-N) or Biotinylated antibody (Cat.-No. AM00042BT-N).
• Reactivities: Canine, Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Phosphopeptide conjugated to KLH.
  Epitope: Phospho-Tyr1197 N A E pY L R V
• Specificity: This antibody specifically interacts with the 1197 - N A E pY L R V motif corresponding to the major autophosphorylation site of Human EGFR.
  Antibody AM00042FC-N does not crossreact with the highly homologous pTyr1248 of acticated erbB2.
• Formulation: PBS containing 0.09% Sodium Azide/PEG and Sucrose.
  Label: FITC
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Purified from serum-free cell culture supernatant by Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
• Conjugation: FITC
• Storage: Store the antibody (aliquote in liquid nitrogen) at -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 1956 Human
• Background: EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases.
  Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity; phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.
• Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1