EGFR (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 13G8]

CAT#: AM00047BT-N

EGFR (C-term) (incl. pos. control) mouse monoclonal antibody, clone 13G8, Biotin

Specifications

Product Data

• Clone Name: 13G8
• Applications: ELISA, IF, IP, WB
• Recommended Dilution: ELISA: 0.05 μg/ml.
  Western Blot: 1 μg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  Immunoprecipitation: 1-10 µg per 10⁶ pervanadate-treated A431 cells.
  Immunocytochemistry: 1-10 μg/ml.
  Luminex.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Peptide conjugated to KLH. Epitope: C-terminus (aa 1165-1186), independent of phosphorylation status.
• Specificity: This antibody specifically recognizes the C-terminus of EGF receptor (aa 1165-1186).
  Recognition is independent of the phosphorylation status at tyrosine 1173.
• Formulation: PBS containing 0.09% Sodium Azide, PEG and Sucrose
  Label: Biotin
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
• Conjugation: Biotin
• Storage: Store the antibody (aliquote in liquid nitrogen) at -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 180 kDa
• Database Link:
  Entrez Gene 1956 Human
• Background: EGF Receptor (EGFR) and erbB2, erbB3, and ErbB4 are members of subclass I of receptor tyrosine kinases. EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y845 by src family kinases. Phosphorylation of Y1045 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1047 by CamKinase II leads to attenuation of kinase activity; phosphorylation of T654 (by PKC) and T669 (by MAPK, p38) interferes with receptor endocytosis/recycling.
• Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
• Note: Included Positive Control: Cell lysate from untreated HepG2 cells
  
  Protocol:

  Positive Control: Cell lysate from untreated HepG2 cells.
  
  Formulation: Lyophilized Cell lysate from serum starved HepG2 cells
  
  Reconstitution: Reconstitute by addition of 200 μl H₂O. After complete solubilization add 200 μl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.

  
  

  Application: The Positive Control is recommended for immunoblot applications.
  20μl of Positive control cell lysate correspond to Ca. 80.000 cells.
  
  Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins.
  
  Note: The lyophilized cell lysates contain SDS and are not recommended fro applications with native proteins such as Immunoprecipitation.
  
  Storage: Aliquote and store frozen.
  Avoid repeated freeze/thaw cycles.
  Shelf life: one year from despatch.