HSP27 (HSPB1) pSer82 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5B9]

CAT#: AM00070BT-N

HSP27 (HSPB1) pSer82 (incl. pos. control) mouse monoclonal antibody, clone 5B9, Biotin


USD 530.00

2 Weeks*

Size
    • 100 ug

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Specifications

Product Data
Clone Name 5B9
Applications IHC, WB
Recommended Dilution Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Positive Control: Cell lysate from EGF-treated HepG2 cells.
Immunohistochemistry on frozen sections.
Reactivities Canine, Human, Mouse, Rat
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Synthetic phosphopeptide conjugated to KLH
Specificity This antibody specifically interacts with hsp27 phosphorylated at serine 82 and does not crossreact with the non-phosphorylated hsp27 nor with unrelated serine-phosphorylated proteins.
Formulation PBS/0.09% Na-Azide/PEG and Sucrose
Label: Biotin
State: Liquid purified IgG
Concentration lot specific
Purification Size exclusion chromatography
Conjugation Biotin
Storage Store the antibody (aliquote in liquid nitrogen) at -80°C.
Avoid repeated freezing and thawing.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Stability Shelf life: one year from despatch.
Background The small heat shock protein hsp27 is constitutively expressed in most cell lines. The expression level is increased in response to environmental stress. Activation of the p38 kinases results in subsequent activation of MAPKAP2 that phosphorylates hsp27 at Ser15, Ser78 and Ser82.
Synonyms Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein, SRP27, HSP25
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.