Insulin Receptor (INSR) pTyr1150/1151 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10C3]

CAT#: AM00075PU-N

Insulin Receptor (INSR) pTyr1150/1151 (incl. pos. control) mouse monoclonal antibody, clone 10C3, Purified

Product Images

Phosphospecificity Whole cell extracts of Control (Lane 1) or Insulin stimulated (Lane 2) MDA-MB-213 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The Immunoblot was probed with Monoclonal InsR antibody 10C3 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).

Specifications

Product Data

• Clone Name: 10C3
• Applications: ELISA, WB
• Recommended Dilution: Western Blot: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  Included Positive Control: Cell lysate from insulin-treated HEK-293 cells.
  ELISA: 0.1 µg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic phosphopeptide conjugated to KLH.
  Epitope: Phosphotyrosine 1150/1151 E T D pY pY R K
• Specificity: This antibody recognizes Insulin receptor phosphorylated at tyrosine residues 1150/1151 and also the IGF1 receptor.
• Formulation: PBS containing 0.09 Sodium Azide/PEG and Sucrose/50% Glycerol
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Size Exclusion Chromatography
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 97 kDa
• Database Link:
  Entrez Gene 3643 Human
• Background: The insulin receptor (InsR) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. The insulin receptor is activated upon binding of the peptide hormone insulin, leading to autophosphorylation of tyrosine residues 1146, 1150, and 1151 in the activation loop of the beta-chain. Additional phosphorylation sites such as tyrosine residues 960, 1316, and 1322 regulate the assembly of signal transduction complexes.
• Synonyms: Insulin Receptor
• Note: Protocol: Positive Control Provided: 293 Insulin treated Positive Control Cell lysate
  
  
  Format: Lyophilized cell lysate from HEK - 293 cells.
  Serum starved cells were treated for 15 min with Insulin.
  
  Reconstitution: Restore by addition of 200 µl H₂0. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
  
  Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.
  
  Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells.
  Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.
  Note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.