JNK2 (MAPK9) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 12C5]

CAT#: AM00079BT-N

JNK2 (MAPK9) (incl. pos. control) mouse monoclonal antibody, clone 12C5, Biotin

Specifications

Product Data

• Clone Name: 12C5
• Applications: ELISA, IHC, WB
• Recommended Dilution: Western Blot: 0.5 μg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  ELISA: 0.05 μg/ml.
  Immunohistochemistry.
  Included Positive Control: Cell lysate from untreated A431 cells (See Protocol).
• Reactivities: Canine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide conjugated to KLH.
  Epitope: N-terminus
• Specificity: This antibody specifically interacts with the N-terminus of SAPK1α/jnk2 kinases.
• Formulation: PBS containing 0.09% Sosium Azide, PEG and Sucrose
  Label: Biotin
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Subsequent Thiophilic Adsorbtion and Size Exclusion Chromatography
• Conjugation: Biotin
• Storage: Store the antibody (aliquote in liquid nitrogen) at -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 54 kDa
• Database Link:
  Entrez Gene 5601 Human
• Background: Stress-activated Protein Kinases (SAPKs) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines. The SAPK1/jnk family consists of 3 isoforms: SAPK1α/jnk2; SAPK1β/jnk3; SAPK1γ/jnk1.
• Synonyms: JNK-55, PRKM9, MAP kinase 9
• Note: Protocol:

  Positive Control Cell Lysate (A431 Untreated)
  
  Formulation: Lyophilized cell lysate from serum starved A431 Cells.
  
  Reconstitution: Restore by Addition of 200 μl Water. After complete solubilization Add 200 μl 2x SDS-PAGE Sample Buffer, mix and incubate at 90°C for 5 mn.
  
  Storage: Aliquote and store frozen.
  Avoid repeated freeze/thaw cycles.
  Shelf life: one year from despatch.
  
  Application: The Positive Control is recommended for Immunoblot (20 μl of Positive Control Cell lysate corresponds to ~ 20.000 Cells).
  Use μl /lane (mini gel) for HRPO/ECL detection of the target proteins.
  
  Please Note: The lyophilized cell lysates contain SDS and are not recomended for the applications with native proteins such as Immunoprecipitation.