JNK2 (MAPK9) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 12C5]

CAT#: AM00079BT-N

JNK2 (MAPK9) (incl. pos. control) mouse monoclonal antibody, clone 12C5, Biotin


USD 465.00

2 Weeks*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name 12C5
Applications ELISA, IHC, WB
Recommended Dilution Western Blot: 0.5 μg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
ELISA: 0.05 μg/ml.
Immunohistochemistry
Included Positive Control: Cell lysate from untreated A431 cells (See Protocol).
Reactivities Canine, Human, Mouse, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Synthetic peptide conjugated to KLH.
Epitope: N-terminus
Specificity This antibody specifically interacts with the N-terminus of SAPK1α/jnk2 kinases.
Formulation PBS containing 0.09% Sosium Azide, PEG and Sucrose
Label: Biotin
State: Liquid purified IgG fraction
Concentration lot specific
Purification Subsequent Thiophilic Adsorbtion and Size Exclusion Chromatography
Conjugation Biotin
Storage Store the antibody (aliquote in liquid nitrogen) at -80°C.
Avoid repeated freezing and thawing.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Stability Shelf life: one year from despatch.
Predicted Protein Size 54 kDa
Background Stress-activated Protein Kinases (SAPKs) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines. The SAPK1/jnk family consists of 3 isoforms: SAPK1α/jnk2; SAPK1β/jnk3; SAPK1γ/jnk1.
Synonyms JNK-55, PRKM9, MAP kinase 9
Note Protocol:

Positive Control Cell Lysate (A431 Untreated)

Formulation: Lyophilized cell lysate from serum starved A431 Cells.

Reconstitution: Restore by Addition of 200 μl Water. After complete solubilization Add 200 μl 2x SDS-PAGE Sample Buffer, mix and incubate at 90°C for 5 mn.

Storage: Aliquote and store frozen.
Avoid repeated freeze/thaw cycles.
Shelf life: one year from despatch.

Application: The Positive Control is recommended for Immunoblot (20 μl of Positive Control Cell lysate corresponds to ~ 20.000 Cells).
Use μl /lane (mini gel) for HRPO/ECL detection of the target proteins.

Please Note: The lyophilized cell lysates contain SDS and are not recomended for the applications with native proteins such as Immunoprecipitation.

Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.