JNK1 (MAPK8) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5D10]

CAT#: AM00081PU-N

JNK1 (MAPK8) (N-term) (incl. pos. control) mouse monoclonal antibody, clone 5D10, Purified

Specifications

Product Data

• Clone Name: 5D10
• Applications: ELISA, IHC, WB
• Recommended Dilution: Western Blot: 0.5 μg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  Positive Control: Cell lysate from untreated HepG2 cells.
  ELISA: 0.05 μg/ml.
  Immunohistochemistry on frozen sections.
• Reactivities: Canine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide conjugated to KLH.
• Specificity: This antibody specifically interacts with the N-terminus of SAPK1γ/jnk1 kinases.
• Formulation: 1 ml 2 x PBS / 0.1 % Na-azide / PEG and Sucrose
  State: Purified
  State: Lyophilized purified IgG
• Reconstitution Method: Restore with 1 ml H2O (15 min, RT).
• Purification: Size exclusion chromatography
• Storage: Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 5599 Human
• Background: Stress-activated Protein Kinases (SAPKs) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines.The SAPK1/jnk family consists of 3 isoforms: SAPK1α/jnk2; SAPK1β/jnk3; SAPK1γ/jnk1.
• Synonyms: Mitogen-activated protein kinase 8, c-Jun N-terminal kinase 1, JNK-46, JNK-1, PRKM8