ERK1 (MAPK3) (pT-E-pY Motif) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 12D4]

CAT#: AM00084BT-N

ERK1 (MAPK3) (pT-E-pY Motif) (incl. pos. control) mouse monoclonal antibody, clone 12D4, Biotin

Specifications

Product Data

• Clone Name: 12D4
• Applications: ELISA, IF, IHC, IP, WB
• Recommended Dilution: Western Blot: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  Positive Control: Cell lysate from pervanadate-treated HepG2 cells.
  ELISA: 0.05 µg/ml.
  Immunoprecipitation: 1 - 10 µg per 10e6 pervanadate-treated A431 or HepG2 cells.
  Immunocytochemistry: 1 - 10 µg/ml.
  Immunohistochemistry on frozen sections.
• Reactivities: Canine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic phosphopeptide conjugated to KLH
• Specificity: This antibody specifically interacts with the pThr - Glu - pTyr motif of activated MAP kinases 1 and 2 (erk1/2). The antibody requires phosphorylation both at the threonine and the tyrosine site and does not interact with the non-phosphorylated form of the protein. Mab MAPK-12D4 shows no crossreaction with activated SAP kinases 1 or 2.
• Formulation: PBS/0.09% Na-Azide/PEG and Sucrose
  Label: Biotin
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Size exclusion chromatography
• Conjugation: Biotin
• Storage: Store the antibody (aliquote in liquid nitrogen) at -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 5595 Human
• Background: Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.
• Synonyms: MAP kinase 3, MAPK 3, ERK-1, ERT2, p44-MAPK, p44-ERK1, PRKM3