ERK2 (MAPK1) (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6G11]

CAT#: AM00085BT-N

ERK2 (MAPK1) (C-term) (incl. pos. control) mouse monoclonal antibody, clone 6G11, Biotin


USD 465.00

2 Weeks*

Size
    • 100 ug

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Specifications

Product Data
Clone Name 6G11
Applications ELISA, IP, WB
Recommended Dilution ELISA: Use at 0.05 µg/ml.
Immunoblotting: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L.
Immunoprecipitation: 1-10 µg/ml per 10e6 pervanadate-treated A431 cells.
Included Positive Control: Cell lysate from untreated HepG2 cells (See Protocols for more details).
Reactivities Canine, Human, Mouse, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Peptide conjugated to KLH
Epitope: C-terminus
Specificity This antibody specifically recognizes the C-terminus of MAP kinase 2 (ERK2). It does not crossreact with MAP kinase 1 (ERK1).
Formulation PBS with 0.09% Sodium Azide/PEG and Sucrose.
Label: Biotin
State: Liquid purified IgG fraction.
Concentration lot specific
Purification Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
Conjugation Biotin
Storage Aliquote and freeze in liquid nitrogen
Antibody can be stored frozen at -80°C up to 1 year.
Thaw aliquots at 37°C. Thawed
aliquots may be stored at 4°C up to 3 months.
Gene Name Homo sapiens mitogen-activated protein kinase 1 (MAPK1), transcript variant 1
Background Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.
Synonyms Mitogen-activated protein kinase 1, p42-MAPK, ERT1, PRKM1, PRKM2, MAP kinase 2, MAPK2, MAPK1
Note Mol. weight: 42 kDa

Protocol: Positive Control Provided.
Cell lysate from untreated HepG2


Description: Cell lysate from untreated HepG2 cells, hepatocellular carcinoma (human)

Format: Lyophilized cell lysate from serum starved HepG2 cells.

Reconstitution:Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.

Storage: Aliquote and store frozen.
Avoid repeated freeze/thaw cycles.

Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.
Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Acute myeloid leukemia, Adherens junction, Alzheimer's disease, Axon guidance, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Non-small cell lung cancer, Oocyte meiosis, Pancreatic cancer, Pathways in cancer, Prion diseases, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, TGF-beta signaling pathway, Thyroid cancer, Toll-like receptor signaling pathway, Type II diabetes mellitus, Vascular smooth muscle contraction, VEGF signaling pathway

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