Phosphotyrosine (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 2C8]

CAT#: AM00124BT-N

Phosphotyrosine (incl. pos. control) mouse monoclonal antibody, clone 2C8, Biotin


USD 465.00

2 Weeks*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name 2C8
Applications ELISA, IP, WB
Recommended Dilution Western Blot: 0.5 μg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
ELISA: 0.1 µg/ml.
Immunoprecipitation: 1 - 10 µg per 106 pervanadate-treated A431 cells.
Reactivities Canine, Human, Mouse, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Synthetic phosphopeptide conjugated to KLH
Specificity Mab PTYR-2C8 recognizes a broad range of tyrosine--phosphorylated proteins in crude cell extracts and may therefore be particularly well-suited for the detection/screening of tyrosine phosphorylated proteins.
Formulation PBS/0.09% Na-Azide/PEG and Sucrose
Label: Biotin
State: Liquid purified IgG
Concentration 0.5 mg/ml
Purification Size exclusion chromatography
Conjugation Biotin
Background Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosine kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via interaction of phosphotyrosine residues with SH2 or PID domains.
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.