Phosphotyrosine (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9H8]

CAT#: AM00127BT-N

Phosphotyrosine (incl. pos. control) mouse monoclonal antibody, clone 9H8, Biotin


USD 465.00

2 Weeks*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name 9H8
Applications ELISA, IP, WB
Recommended Dilution ELISA: Use at 0.05 µg/ml.
Immunoblotting: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L.
Immunoprecipitation: Use at 1-10 µg per 106 pervanadate-treated A431 cells.
Included Positive Control: Phosphotyrosine MW standard.
Reactivities Canine, Human, Mouse, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Synthetic phosphopeptide conjugated to KLH
Epitope: ...R-G-pY-V-P...
Specificity This antibody recognizes Phosphotyrosine in the context of the surrounding amino acids, tolerating positively charged amino acids N-terminal to phosphotyrosine.
Formulation PBS with 0.09% Sodium Azide/PEG and Sucrose
Label: Biotin
State: Liquid purified IgG fraction
Concentration 0.5 mg/ml
Purification Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation Biotin
Background Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosine kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via interaction of phosphotyrosine residues with SH2 or PID domains.
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.