Phosphotyrosine (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9H8]

CAT#: AM00127BT-N

Phosphotyrosine (incl. pos. control) mouse monoclonal antibody, clone 9H8, Biotin

Specifications

Product Data

• Clone Name: 9H8
• Applications: ELISA, IP, WB
• Recommended Dilution: ELISA: Use at 0.05 µg/ml.
  Immunoblotting: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L.
  Immunoprecipitation: Use at 1-10 µg per 10⁶ pervanadate-treated A431 cells.
  Included Positive Control: Phosphotyrosine MW standard.
• Reactivities: Canine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic phosphopeptide conjugated to KLH
  Epitope: ...R-G-pY-V-P...
• Specificity: This antibody recognizes Phosphotyrosine in the context of the surrounding amino acids, tolerating positively charged amino acids N-terminal to phosphotyrosine.
• Formulation: PBS with 0.09% Sodium Azide/PEG and Sucrose
  Label: Biotin
  State: Liquid purified IgG fraction
• Concentration: 0.5 mg/ml
• Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
• Conjugation: Biotin
• Background: Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosine kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via interaction of phosphotyrosine residues with SH2 or PID domains.