RAF1 pSer621 Mouse Monoclonal Antibody [Clone ID: 6B4]

CAT#: AM00131PU-N

RAF1 pSer621 mouse monoclonal antibody, clone 6B4, Purified

Specifications

Product Data

• Clone Name: 6B4
• Applications: ELISA, WB
• Recommended Dilution: Western Blot: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  ELISA: 0.05 µg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Synthetic phosphopeptide conjugated to KLH.
  Epitope: Phosphoserine 621
• Specificity: This antibody Clone 6B4 specifically recognizes c-raf phosphorylated at Serine 621.
  The antibody is an important tool to analyse the contribution of the Serine 621 phosphorylation site to c-raf activity.
• Formulation: 1ml 2 x PBS containing 0.09% Sodium Azide / PEG and Sucrose
  State: Purified
  State: Lyophilized purified IgG fraction from serum-free cell Culture Supernatant
• Reconstitution Method: Restore with 1 ml H2O (15 min, RT).
• Purification: Size Exclusion Chromatography
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 74 kDa
• Gene Name: Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1)
• Database Link:
  Entrez Gene 5894 Human
• Background: The c-raf protein is an important kinase in the transmission of extracellular stimuli to the nucleus. Mitogenic stimulaton of cells induces hyperphosphorylation of c-raf in multiple serine, threonine and tyrosine residues. Phosphorylation of serines 43, 259 and 621 has been implicated in the negative regulation of raf. Phosphorylation of serine 43 interferes with ras binding, serine 259 and serine 621 represent binding sites for the 14-3-3 proteins.
• Synonyms: C-RAF, Raf-1, cRaf

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Vascular smooth muscle contraction, VEGF signaling pathway