Scramblase 1 (PLSCR1) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 1E9]

CAT#: AM00139PU-N

Scramblase 1 (PLSCR1) (N-term) (incl. pos. control) mouse monoclonal antibody, clone 1E9, Purified

Product Images

Detection of Endogenous Scramblase 1. Whole cell lysates of serum starved tumor cells were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab Scram1-1E9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK-293 Lane 4: SY5Y Lane 5: Jurkat Lane 6: U937 Lane 7: K562 Lane 8: THP

Specifications

Product Data

• Clone Name: 1E9
• Applications: WB
• Recommended Dilution: Western Blot: 1 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide conjugated to KLH.
• Specificity: This antibody specifically recognizes the N-terminus of scramblase-1 at 35 kDa.
• Formulation: 1 ml PBS containing 0.09% Sodium Azide as preservative, PEG and Sucrose.
  State: Purified
  State: Lyophilized purified IgG fraction.
• Reconstitution Method: Restore with 1 ml H2O (15 min, RT).
• Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
• Storage: Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens phospholipid scramblase 1 (PLSCR1)
• Database Link:
  Entrez Gene 5359 Human
• Background: Scramblase-1 is a calcium-binding protein that can be induced by interferon and growth factors (e.g. epidermal growth factor EGF). Depending on its palmitoylation state, scramblase-1 either inserts into the plasma membrane or binds DNA in the nucleus. Scramblase-1 is a substrate for src kinases.
• Synonyms: Phospholipid scramblase 1, PL scramblase 1, MmTRA1b
• Note: Protocol: Positive Control Cell Lysate
  HepG2 untreated
  
  Size Sufficient: 20 mini-gel lanes.
  
  Formulation: Lyophilized cell lysate from serum starved HepG2 cells.
  
  Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
  
  Storage: Aliquote and store frozen.
  Avoid repeated freeze/thaw cycles.
  
  Application: The positive control cell lysate is recommended for Immunoblot applications.
  20 µl of positive control cell lysate correspond to ca. 80.000 cells.
  Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.
  
  Please Note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency