Scramblase 1 (PLSCR1) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 1E9]

CAT#: AM00139PU-N

Scramblase 1 (PLSCR1) (N-term) (incl. pos. control) mouse monoclonal antibody, clone 1E9, Purified


USD 465.00

2 Weeks*

Size
    • 100 ug

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Specifications

Product Data
Clone Name 1E9
Applications WB
Recommended Dilution Western Blot: 1 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Synthetic peptide conjugated to KLH.
Specificity This antibody specifically recognizes the N-terminus of scramblase-1 at 35 kDa.
Formulation 1 ml PBS containing 0.09% Sodium Azide as preservative, PEG and Sucrose.
State: Purified
State: Lyophilized purified IgG fraction.
Reconstitution Method Restore with 1 ml H2O (15 min, RT).
Purification Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
Storage Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Gene Name Homo sapiens phospholipid scramblase 1 (PLSCR1)
Background Scramblase-1 is a calcium-binding protein that can be induced by interferon and growth factors (e.g. epidermal growth factor EGF). Depending on its palmitoylation state, scramblase-1 either inserts into the plasma membrane or binds DNA in the nucleus. Scramblase-1 is a substrate for src kinases.
Synonyms Phospholipid scramblase 1, PL scramblase 1, MmTRA1b
Note Protocol: Positive Control Cell Lysate
HepG2 untreated

Size Sufficient: 20 mini-gel lanes.

Formulation: Lyophilized cell lysate from serum starved HepG2 cells.

Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.

Storage: Aliquote and store frozen.
Avoid repeated freeze/thaw cycles.

Application: The positive control cell lysate is recommended for Immunoblot applications.
20 µl of positive control cell lysate correspond to ca. 80.000 cells.
Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.

Please Note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data
Protein Families Druggable Genome, Stem cell - Pluripotency

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