SHC (SHC1) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 24E4]

CAT#: AM00143PU-N

SHC (SHC1) (N-term) (incl. pos. control) mouse monoclonal antibody, clone 24E4, Purified

Product Images

Detection of endogenous p66shc: Whole cell lysates of serum starved tumor cells (ca. 20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab shc/p66-24E4 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12 Lane 7: CMT 93 Lane 8: Neuro 2A Lane 9: 3T3

Specifications

Product Data

• Clone Name: 24E4
• Applications: WB
• Recommended Dilution: Western Blot: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  Included Positive Control: Cell lysate from untreated Neuro 2A cells (See Protocol below).
• Reactivities: Canine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide conjugated to KLH.
  Epitope: N-terminus.
• Specificity: This antibody specifically recognizes the N- terminus of Shc/p66.
• Formulation: PBS containing 0.09% Sodium Azide, PEG and Sucrose, 50% Glycerol
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 66 kDa
• Database Link:
  Entrez Gene 6464 Human
• Background: Mammalian cells can express three alternatively spliced isoforms of the shc adaptor protein: shc/p46, shc/p52 and shc/p66. shc/p66 contains a unique N-terminal protein domain. In addition to tyrosine phosphorylation of Tyr 239/240 and/or Tyr 317, shc/p66 is phosphorylated at serine 36, e.g. in response to EGF. Serine phosphorylation of shc/p66 impairs its ability to bind to the activated EGF receptor thus inhibiting EGF receptor downstream signalling pathways.
• Synonyms: SHC-transforming protein 1, SHC-transforming protein A, SHC-transforming protein 3, SHCA
• Note: Protocol: Included Positive Control: Cell lysate from untreated Neuro 2A cells.
  
  Formulation: Lyophilized cell lysate from serum starved Neuro 2A cells.
  
  Reconstitution: Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 54 min.
  
  Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells.
  Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins.
  Please note: The cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation!
  
  Storage: Aliqot and store at -20°C.
  Avoid repeated freezing and thawing.