SRC pTyr416 Mouse Monoclonal Antibody [Clone ID: 9A6]

CAT#: AM00145BT-N

SRC pTyr416 mouse monoclonal antibody, clone 9A6, Biotin


USD 530.00

2 Weeks*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name 9A6
Applications ELISA, FC, IF, WB
Recommended Dilution Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
ELISA: 0.05 µg/ml.
Flow Cytometry.
Immunocytochemistry.
Reactivities Human, Mouse
Host Mouse
Isotype IgG3
Clonality Monoclonal
Immunogen Synthetic phosphopeptide conjuagted to KLH
Specificity This antibody specifically interacts with activated src phosphorylated at Tyr 416 and with src family kinases phosphorylated at the corresponding Tyr in the activation loop. The antibody does not crossreact with the non-phosphorylated form of src nor with unrelated phosphorylation sites.
Formulation 0.5 mg/ml in PBS/0.09% Na-Azide/PEG and Sucrose.
Label: Biotin
State: Liquid purified IgG fraction.
Purification Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation Biotin
Storage Store the antibody (Aliquote and freeze in liquid nitrogen)at -80°C.
Avoid repeated freezing and thawing.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Stability Shelf life: one year from despatch.
Background Src is the best understood member of a family of nine tyrosine kinases regulating cellular responses to extracellular stimuli. Src becomes activated upon dephosphorylation of Tyr 527, leading to a conformational change of the src molecule towards an open, active conformation which allows binding to other proteins via the SH2 and SH3 domain. Activated src phosphorylates a variety of substrates involved in the mitogenic signalling pathway, cytoskeletal organization, cell-substrate adhesion, cell-cell adhesion, cell-cell communication, and RNA processing.
Synonyms c-Src, pp60c-src
Note Molecular weight of antigen: 60 kDa
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.