VASP pSer157 Mouse Monoclonal Antibody [Clone ID: 5C6]

CAT#: AM00155BT-N

VASP pSer157 mouse monoclonal antibody, clone 5C6, Biotin

Specifications

Product Data

• Clone Name: 5C6
• Applications: ELISA, FC, IF, IP, WB
• Recommended Dilution: Western blot: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  ELISA: 0.05 µg/ml.
  Immunoprecipitation: 1-10 µg per 10e6 pervanadate-treated A431 cells.
  Immunocytochemistry: 1-10 µg/ml (VASP-5C6 may tolerate 0.5% formaldehyde fixation).
  Flow Cytometry.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic phosphopeptide conjugated to KLH.
  Epitope: Phosphoserine 157
• Specificity: This antibody recognizes VASP only, when Ser 157 is phosphorylated, a site preferred by cAMP-dependent protein kinase (PKA). The antibody does not crossreact with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, antibody VASP-5C6 is able to monitor the phosphorylation state of VASP serine 157 as well as PKA activity.
• Formulation: PBS with 0.09% Sodium Azide, PEG and Sucrose
  Label: Biotin
  State: Liquid purified IgG fraction from serum-free cell culture supernatant
• Concentration: lot specific
• Purification: Thiophilic Adsorption and Size Exclusion Chromatography
• Conjugation: Biotin
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 7408 Human
• Background: VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling pathways. VASP is expressed in almost all human and animal cell lines; particularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament
• Synonyms: Vasodilator-stimulated phosphoprotein