VASP pSer157 Mouse Monoclonal Antibody [Clone ID: 5C6]

CAT#: AM00155FC-N

VASP pSer157 mouse monoclonal antibody, clone 5C6, FITC

Specifications

Product Data

• Clone Name: 5C6
• Applications: FC, IF
• Recommended Dilution: Flow Cytometry.
  Immunocytochemistry: 1-10 µg/ml (may tolerate 0.5% Formaldehyde fixation).
  For ELISA, Immunoblotting and Immunoprecipitation use Purified antibody Cat.-No AM00155PU-N and Biotin conjugated Cat.-No AM00155BT-N.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Phosphopeptide conjugated to KLH.
  Epitope: Phosphoserine 157
• Specificity: This antibody recognizes VASP only, when Ser157 is phosphorylated, a site preferred by cAMP-dependent protein kinase (PKA).
  The antibody does not crossreact with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, this antibody is able to monitor the phosphorylation state of VASP Serine157 as well as PKA activity.
• Formulation: 2 x PBS containing 0.09% Sodium Azide, PEG and Sucrose
  Label: FITC
  State: Liquid purified IgG fraction from serum-free cell culture supernatant
  Label: Fluorescein Isothiocyanate
• Concentration: lot specific
• Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 46/50 kDa
• Database Link:
  Entrez Gene 7408 Human
• Background: VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling pathways. VASP is expressed in almost all human and animal cell lines; particularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation.
• Synonyms: Vasodilator-stimulated phosphoprotein