VASP pSer239 Mouse Monoclonal Antibody [Clone ID: 16C2]

CAT#: AM00156FC-N

VASP pSer239 mouse monoclonal antibody, clone 16C2, FITC

Specifications

Product Data

• Clone Name: 16C2
• Applications: FC, IF
• Recommended Dilution: Flow Cytometry.
  Immunocytochemistry: 1-10 µg/ml (may tolerate 0.5% Formaldehyde fixation).
  For Immunoblotting and Immunoprecipitation use Cat.-No AM00156PU-N and AM00156BT-N.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic phosphopeptide conjugated to KLH.
  Epitope: Phosphoserine 239
• Specificity: This antibody recognizes VASP only, when Ser239 is phosphorylated, a site preferred by cGMP-dependent protein kinase (PKG) but also used by cAMP-dependent protein kinase (PKA).
  This antibody does not crossreact with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, The antibody is able to monitor the phosphorylation state of VASP Serine239 as well as PKA activity.
• Formulation: 2 x PBS containing 0.09% Sodium Azide / PEG and Sucrose
  Label: FITC
  State: Liquid purified IgG fraction from serum-free cell culture supernatant
  Label: Fluorescein Isothiocyanate
  Molar radio: DOL = 2.9
• Concentration: lot specific
• Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
• Conjugation: FITC
• Storage: Aliquote and freeze (in liquid nitrogen at -20°C to -80°C).
  Thaw aliquots at 37°C.
  Thawed aliquots may be stored at 2-8°C up to 3 months.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 46/50 kDa
• Database Link:
  Entrez Gene 7408 Human
• Background: VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling pathways. VASP is expressed in almost all human and animal cell lines; particularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament format
• Synonyms: Vasodilator-stimulated phosphoprotein