ROCK1 Mouse Monoclonal Antibody [Clone ID: 12M03]

CAT#: AM00191PU-N

ROCK1 mouse monoclonal antibody, clone 12M03, Purified

Specifications

Product Data

• Clone Name: 12M03
• Applications: WB
• Recommended Dilution: Western Blot: 1-4 μg/ml.
  Jurkat cell lysate treated with Camptothecin (2µM for 6-8 hours) can be used as a Positive Control.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Synthetic peptide corresponding to the Caspase-3 cleavage site
• Specificity: The antibody has been selected for its ability to recognize the cleaved ROCK-1.
  It does not recognize the full length protein.
• Formulation: PBS, pH 7.2 containing 50% Glycerol, 1% BSA and 0.02% Thimerosal
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Protein G Chromatography
• Storage: Upon receipt, store (in aliquots) at -20°C to -80°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 6093 Human
• Background: ROCK-1 (Rho-associated coiled coil-containing protein kinase 1) has been found to be a new caspase-3 substrate. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain. During apoptosis, ROCK-1 is cleaved by caspase-3 at the conserved DETD1113/G sequence and its C-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3 mediated cleavage and activation of ROCK-1 induces phosphorylation of MLC and membrane blebbing, the early events in the execution phase of apoptosis.
• Synonyms: ROCK-1, p160ROCK, NY-REN-35