MDM2 (154-167) Mouse Monoclonal Antibody [Clone ID: SMP14]

CAT#: AM00224PU-N

MDM2 (154-167) mouse monoclonal antibody, clone SMP14, Purified

Product Images

Formalin-fixed, paraffin-embedded human breast carcinoma stained with MDM2 Antibody.

Specifications

Product Data

• Clone Name: SMP14
• Applications: IHC, IP, WB
• Recommended Dilution: Immunoprecipitation.
  Immunohistochemistry on Frozen and Resin Sections.
  Immunohistochemistry on Paraffin Sections: 1/200-1/500.
  Antigen retrieval using heat treatment prior to staining of paraffin sections is required. EDTA pH 8.0 is recommended for this purpose.
  Recommended Positive Control: Breast Carcinoma.
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A Synthetic peptide corresponding to amino acids 154-167 of Human MDM2 protein.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.
• Specificity: This antibody recognizes the 90kD MDM2 protein, which forms a complex with p53 leading to p53 inhibition. May also acts to stimulate cell proliferation via its induction of transcription factors such as E2F1 and DPI.
  MDM2 is over expressed in a range of human malignancies, including soft tissue sarcomas, breast cancer and bladder cancer.
  Cellular Localization: nuclear.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 4193 Human
• Background: Human homolog of Murine Double Minute-2 (MDM2) and p53 function in mutual regulation: activation of p53 results in expression of MDM2 which consequently inhibits the ability of wild type p53 to stimulate expression of target genes. MDM2 is frequently overexpressed in the nuclei of soft tissue sarcomas, and to a much lesser extent, in a wide variety of malignancies of bladder, breast, testes, cervix, and glial cells.
• Synonyms: p53-binding protein Mdm2, Oncoprotein Mdm2, Double minute 2 protein, Hdm2
• Note: Predicted Molecular weight: 55 kDa.