Myog Mouse Monoclonal Antibody [Clone ID: F5D]

CAT#: AM00317SU-N

Myog mouse monoclonal antibody, clone F5D, Supernatant

Product Images

Formalin-Fixed, Paraffin-Embedded Human rhabdomyosarcoma stained with Myogenin antibody Cat.-No. AM00317SU-N using peroxidase conjugate and DAB chromogen. Note nuclear staining of tumor cells.

Specifications

Product Data

• Clone Name: F5D
• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot.
  Immunofluorescence.
  Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Positive Control: Rhabdomyosarcoma or Wilm's tumors.
• Reactivities: Feline, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant protein containing Rat Myogenin amino acids 30-224
• Specificity: Reacts with a 34 kD protein known as Myogenin.
  The antibody can immunoprecipitate protein-DNA complex.
  Cellular Localization: Nuclear.
• Formulation: State: Supernatant
  State: Liquid Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
• Purification: Protein G Chromatography.
• Gene Name: Rattus norvegicus myogenin (Myog)
• Database Link:
  Entrez Gene 4656 HumanEntrez Gene 17928 MouseEntrez Gene 29148 Rat
• Background: Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Transfection of myogenin into multipotential mesodermal cells have been shown to convert the mesodermal cells to myoblasts. Expression of Myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.
• Synonyms: MYOG, MYF4, Myogenic factor 4, BHLHC3